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A kind of SNP molecular marker related to multiple lambs in sheep and its application

A molecular marker and sheep technology, applied in recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of difficult high-throughput automatic measurement, low throughput, and numerous procedures, so as to improve fecundity , low cost and easy to use

Active Publication Date: 2022-05-31
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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Problems solved by technology

[0006] However, there is currently no report on the specific role of the BAD gene in the breeding process of sheep. Most of the traditional genotype detection methods use PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) and PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism). ) detection methods, these methods have low throughput and various procedures, and it is difficult to achieve high-throughput automated determination

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  • A kind of SNP molecular marker related to multiple lambs in sheep and its application
  • A kind of SNP molecular marker related to multiple lambs in sheep and its application
  • A kind of SNP molecular marker related to multiple lambs in sheep and its application

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Embodiment 1

[0045] In the present embodiment, all reagents and instruments are purchased from Beijing Genenode Biotech Co., Ltd., wherein the specific selection of reagents and instruments is shown in Table 1:

[0046] Table 1 Experimental reagents and instruments

[0047]

[0048]

[0049] 1. Experimental animals

[0050] The experimental sheep used in the present invention were healthy small-tailed Han sheep from Yuncheng County, Shandong Province, and a total of 379 sheep were selected.

[0051] 2. Extraction of genomic DNA of sheep to be tested

[0052] 1 ml of jugular venous blood was collected from all individuals in the above-mentioned sheep population, and treated with EDTA for anticoagulation. The erythrocyte lysate was used to lyse the erythrocytes without DNA, and then the cells were lysed with the nucleus lysate to release the genomic DNA, and then the protein precipitation solution was used to selectively precipitate to remove the protein. Finally, the purified genomi...

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Abstract

The invention provides a SNP molecular marker related to the multiple-lamb character of sheep, a detection kit and application thereof, and belongs to the technical field of sheep SNP molecular markers. The SNP is the 41895346bp site on chromosome 21 (XM_004019650.3, based on the sheep genome sequence information version number Oar_v3.1, December 2012), and the dominant allele of the site is selected by typing the site Gene, to increase the average litter size of a litter of ewes in sheep. The present invention further uses Sequenom technology to detect the single nucleotide type of this site, determine the BAD genotype of sheep, and select TC heterozygous individuals and CC homozygous individuals with high litter size traits to stay for breeding, realizing large-scale, Automated detection has potential application value for large-scale molecular breeding of sheep.

Description

technical field [0001] The invention relates to a molecular marker detection technology, in particular to a SNP molecular marker related to sheep polyps and applications thereof. Background technique [0002] Sheep (Ovis aries) are commonly raised animals and are raised all over the world to provide humans with products such as meat and fur. The litter size trait is one of the most important economic traits in sheep. Because it is controlled by micro-effect polygenes and has low heritability, it is difficult to rapidly improve it by conventional breeding methods. Molecular technology can effectively and quickly improve its genetic progress. Therefore, Identifying major genes or molecular genetic markers associated with litter size is the key to modern molecular breeding. [0003] BAD protein is a member of the B cell lymphoma-2 (Bcl-2) protein family. Bcl-2-associated agonist of cell death (BAD) belongs to the Bcl-2 family and is a pro-apoptotic protein containing only BH3...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/156C12Q2600/124C12Q2531/113C12Q2521/525C12Q2565/627
Inventor 储明星曹晓涵贺小云刘秋月
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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