Method for inhibiting tumor cell growth by utilizing illumination and experimental device

A tumor cell and experimental device technology, applied in the field of methods and experimental devices, to achieve the effect of reducing heat

Active Publication Date: 2021-03-12
ZHEJIANG UNIV
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  • Application Information

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Problems solved by technology

[0010] The purpose of the present invention is to provide a method and an experimental device for inhibiting the growth of tumor cells by using light to solve the problems of the expression of light-sensitive proteins and light exposure to tumor cells in the prior art

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  • Method for inhibiting tumor cell growth by utilizing illumination and experimental device
  • Method for inhibiting tumor cell growth by utilizing illumination and experimental device
  • Method for inhibiting tumor cell growth by utilizing illumination and experimental device

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Embodiment Construction

[0039] The following will be combined with the accompanying drawings in the embodiments of the present invention; the technical solutions in the embodiments of the present invention will be clearly and completely described; obviously; the described embodiments are only some embodiments of the present invention; rather than all embodiments, based on The embodiments in the present invention; all other embodiments obtained by those skilled in the art without creative work; all belong to the protection scope of the present invention.

[0040] Optogenetics is a multidisciplinary technology that integrates optics, software control, gene manipulation technology, and electrophysiology. Since 2005, the laboratory of Karl Deisseroth at Stanford University has achieved the regulation of neurons by expressing light-sensitive proteins in neurons in response to light stimulation of different wavelengths, announcing that humans have formally possessed the tools to precisely manipulate neurons...

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Abstract

The invention relates to the field of optogenetics and neurosciences, in particular to a method for inhibiting tumor cell growth by utilizing illumination and an experimental device. The method comprises the following steps: S1, pumping Cl- into tumor cells stably expressing eNpHR3.0 channel protein after irradiating the tumor cells with yellow light with a specific wavelength of 590nm, thus stably expressing the channel protein on a cell membrane of a tumor cell; s2, putting the tumor cells stably expressing the channel protein into a culture device, and covering the culture device with a culture device cover; s3, providing illumination with a specific wavelength of 590nm, activating the channel protein, and pumping Cl- into the cells to increase the concentration of Cl- in the cells; andS4, detecting the proliferation activity of the tumor cells to acquire an inhibited conclusion of the cell activity by detecting indexes such as the proliferation cell cycle, apoptosis and exosome secretion amount of the tumor cells. Through illumination, the concentration of Cl- in the tumor cells stably expressing the channel protein is increased, and the growth of the tumor cells is effectively inhibited.

Description

technical field [0001] The invention relates to the fields of optogenetics and neuroscience, in particular to a method and an experimental device for inhibiting tumor cell proliferation by using light. Background technique [0002] In recent years, although optogenetics technology has made remarkable achievements, there are still some problems that need our attention. [0003] First of all, some people think that the achievements of optogenetics in the field of neuroscience can translate its theory to the field of neuro-oncology. The introduction of such exogenous excitatory or inhibitory stimuli to neural circuits can make neurons respond beyond the physiological range. In such cases, neural circuits undergo unnatural changes, leading to incorrect physiological conclusions. [0004] Second, light-sensitive protein expression and illumination are not uniform across neuronal populations, with the result that heterogeneity can occur in the magnitude and extent of optogenetic ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N13/00C12N5/09C12M3/00C12M1/02
CPCC12N13/00C12N5/0693C12M23/12C12M23/22C12M23/38C12M31/02C12M41/18C12N2529/10
Inventor 张楠杜嵩相学平祝向东
Owner ZHEJIANG UNIV
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