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Preparation and application of biosensor based on N-graphene nanobelt-gold platinum nanocluster

A graphene nanoribbon and biosensor technology, applied in the field of biosensors, can solve the problems of high requirements for testing personnel, cumbersome operation process, complex instruments and equipment, etc., and achieve the effects of shortened detection time, simple preparation and wide application range

Pending Publication Date: 2021-03-09
SHANDONG INST FOR PROD QUALITY INSPECTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the shortcomings of traditional detection methods such as complex equipment, cumbersome operation process, high requirements for detection personnel, and high detection cost, the present invention proposes a method for simultaneous detection of ascorbic acid and dopamine with high sensitivity, good selectivity, good reproducibility, simple operation, and low cost. , the preparation method of the biosensor of uric acid

Method used

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  • Preparation and application of biosensor based on N-graphene nanobelt-gold platinum nanocluster
  • Preparation and application of biosensor based on N-graphene nanobelt-gold platinum nanocluster
  • Preparation and application of biosensor based on N-graphene nanobelt-gold platinum nanocluster

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Preparation of N-graphene nanobelt-gold platinum nanocluster composite

[0042] (1) Mix 0.1g N-multiwalled carbon nanotubes with 30mL H 2 SO 4 / H 3 PO 4 (9:1) The mixed solution was placed in a microwave reaction bottle, and the temperature of the microwave reactor was controlled at 110 °C for 2 min. After cooling for 10min, add 2g KMnO to the reaction solution 4 , the microwave temperature was controlled at 65°C for 4 min. Cool to room temperature, wash thoroughly with ultrapure water until neutral. The obtained black solid, that is, N-graphene nanobelts, was dried under vacuum at 35°C-40°C.

[0043] (2) Mix 1mL 10mg / mL PVA solution with 200mL 0.25mM AuCl - 4The solutions were mixed. Stir vigorously for 1 h, then add 5 mL of 0.1 M fresh trisodium citrate solution dropwise, and stir the solution for 2 h to generate Au colloids (particle size 3.0 nm). Add 10 mL of 1 mM K to the pre-prepared gold colloidal solution 2 PtCl 6 and a solution of 5 g / L PVP. After b...

Embodiment 2

[0050] The difference between this embodiment and Example 1 is that the preparation of the biosensor of N-graphene nanoribbons-gold platinum nanoclusters:

[0051] (1) Use A1 2 o 3 A glassy carbon electrode with a diameter of 4 mm was powder-polished and rinsed with ultrapure water.

[0052] (2) Take 10 µL of the stock solution of the N-graphene nanoribbon-AuPt nanocluster composite and drop it on the glassy carbon electrode, and then dry it to prepare the bio sensor.

Embodiment 3

[0054] Preparation of N-graphene nanobelt-gold platinum nanocluster composite

[0055] (1) Mix 0.5g N-multiwalled carbon nanotubes with 200mL H 2 SO 4 / H 3 PO 4 (9:1) The mixed solution was placed in a microwave reaction bottle, and the temperature of the microwave reactor was controlled at 140 °C for 3 min. After cooling for 15min, add 10g KMnO to the reaction solution 4 , the microwave temperature was controlled at 85°C for 10 min. Cool to room temperature, wash thoroughly with ultrapure water until neutral. The obtained black solid, that is, N-graphene nanobelts, was dried in vacuum at 40° C. for 1 h.

[0056] (2) Mix 0.5mL 10mg / mL PVA solution with 170mL 0.25mM AuCl - 4 The solutions were mixed. Stir vigorously for 1h, then add 12mL of 0.1M fresh trisodium citrate solution dropwise, and stir the solution for 2h to generate Au colloids (average particle size 6.0nm). Add 50 mL of 1 mM K to the pre-prepared gold colloidal solution 2 PtCl 6 and a solution of 5 g / L P...

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Abstract

The invention belongs to the technical field of biosensors and relates to preparation and application of a biosensor based on an N-graphene nanobelt-gold-platinum nano-cluster. A glassy carbon electrode is used as a working electrode, and an N-graphene nanobelt-gold-platinum nano-cluster compound is used for modification. The glassy carbon electrode is adopted, the price is low, the electrochemical technology and the biosensing technology are combined, selectivity of the sensor is improved, sample pretreatment is not needed for a sample, the detection time is shortened, meanwhile, detection cost is reduced, and the detection method is rapid, low in price and high in sensitivity and selectivity.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and relates to the preparation and application of a biosensor based on N-graphene nanobelt-gold platinum nanoclusters. Background technique [0002] Ascorbic acid (AA) is a water-soluble vitamin (vitamin C), which is indispensable in human life activities and plays a regulatory role in redox metabolic reactions. The human body cannot produce vitamin C by itself. If there is a large amount of vitamin C deficiency in the body, scurvy can be caused. [0003] Dopamine (DA) is synthesized and secreted from the human brain and can affect people's emotions. It is a neurotransmitter in the human brain. This brain secretion is mainly responsible for the brain's lust, which conveys information of excitement and happiness, and is also related to addiction. Therefore, exploring the role of dopamine concentration in certain diseases (depression, Parkinson's disease, Alzheimer's disease, etc.) is very imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327G01N27/48
CPCG01N27/308G01N27/3278G01N27/48
Inventor 张娟赵丽芳滕龙于燕萍孙文丹邹惠玲于晓菲田静刘鹏飞齐云
Owner SHANDONG INST FOR PROD QUALITY INSPECTION
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