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A kind of living preservation method of lily

A preservation method and technology for lily, applied in the field of plant biology, can solve the problems of short storage time in vitro, affecting the quality of lily, etc., and achieve the effects of long-term preservation, reduction of preservation cost, and improvement of survival rate.

Active Publication Date: 2022-04-29
INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The in vitro storage time of culture is short, and the quality of lily is affected by subculture for many times. Using this invention, lily can be preserved for at least 500 days

Method used

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  • A kind of living preservation method of lily

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A method for preserving lilies in vivo, comprising the following steps:

[0042] Step 1: Select lily bulbs with a diameter of 6 cm, and peel off the outer scales of the bulbs as explants for living preservation;

[0043] Step 2: Sterilize the explants. Put the explants into a sterile container on the ultra-clean table, soak them in 75% alcohol for 2 minutes, take out the explants, and rinse them with sterile water for 5 times. Then use 0.1HgCl 2 Soak in the solution for 20 minutes, then rinse with sterile water for 5 times, each time for 3 minutes, and finally dry the water with sterile filter paper;

[0044] Step 3: placing the sterilized explants into the prepared preservation medium for living preservation;

[0045] The preservation medium is:

[0046] Improved MS+inositol 0.2g / L+mannitol 500mg / L+silver nitrate 0.5mg / L

[0047] The improved MS formula is: the content of major elements is reduced by 50%, and the rest of the ingredients remain unchanged;

[0048] ...

Embodiment 2

[0055] A method for preserving lilies in vivo, comprising the following steps:

[0056] Step 1: Select lily bulbs with a diameter of 6 cm, and peel off the outer scales of the bulbs as explants for living preservation;

[0057] Step 2: Sterilize the explants. Put the explants into a sterile container on the ultra-clean table, soak them in 75% alcohol for 2 minutes, take out the explants, and rinse them with sterile water for 5 times. Then use 0.1HgCl 2 Soak in the solution for 20 minutes, then rinse with sterile water for 5 times, each time for 3 minutes, and finally dry the water with sterile filter paper;

[0058] Step 3: placing the sterilized explants into the prepared preservation medium for living preservation;

[0059] The preservation medium is:

[0060]Improved MS+inositol 0.15g / L+mannitol 500mg / L+silver nitrate 0.2mg / L

[0061] The improved MS formula is: the content of major elements is reduced by 50%, and the rest of the ingredients remain unchanged;

[0062] ...

Embodiment 3

[0065] A method for preserving lilies in vivo, comprising the following steps:

[0066] Step 1: Select lily bulbs with a diameter of 6 cm, and peel off the outer scales of the bulbs as explants for living preservation;

[0067] Step 2: Sterilize the explants. Put the explants into a sterile container on the ultra-clean table, soak them in 75% alcohol for 2 minutes, take out the explants, and rinse them with sterile water for 5 times. Then use 0.1HgCl 2 Soak in the solution for 20 minutes, then rinse with sterile water for 5 times, each time for 3 minutes, and finally dry the water with sterile filter paper;

[0068] Step 3: placing the sterilized explants into the prepared preservation medium for living preservation;

[0069] The preservation medium is:

[0070] Improved MS+inositol 0.15g / L+mannitol 500mg / L+silver nitrate 0.6mg / L

[0071] The improved MS formula is: the content of major elements is reduced by 50%, and the rest of the ingredients remain unchanged;

[0072]...

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Abstract

The invention discloses a living preservation method of lilies, which comprises the following steps: step 1: selecting lily bulbs with a diameter of 3-10 cm, and stripping the outer scales of the bulbs as explants for living preservation; step 2: explants Sterilize; step 3: place the sterilized explants into the prepared preservation medium for living preservation; the preservation medium is: improved MS+inositol 0.15-0.35g / L+mannitol 400-700mg / L L+silver nitrate 0.2-0.6mg / L, the improved MS formula is: the content of macroelements is reduced by 40%-60%, and the remaining ingredients are unchanged; Optimum culture conditions improve the survival rate of lily live preservation, and the operation is simple and low in cost, the preservation conditions are simplified and the preservation cost is reduced. It has laid a reliable foundation for the realization of large-scale living lily preservation.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a living preservation method of lilies. Background technique [0002] Lily is a perennial bulbous herbaceous plant of the genus Lilium in the monocot class Liliaceae (Liliaccae). The ornamental value, edible value and medicinal value of lily play a decisive role in the market. At the same time, China is a big country in the output of unique varieties of lily. However, many lily cultivars are on the verge of extinction due to environmental pollution, pest poisoning and other harsh environments; therefore, the current focus of attention is to properly preserve the existing germplasm resources of lily. [0003] Conservation in vitro refers to the preservation of seeds, pollen, roots, spikes, buds and other reproductive materials that have left the parent body of germplasm resources. The in vitro preservation methods of lilies include tissue culture in vitro preservat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N3/00A01H4/00
CPCA01N3/00A01H4/001A01H4/008
Inventor 马怀林张跃华贾振宇高俊刚刘思泱刘亚玲高旭屈璐璐王乌恩巴图
Owner INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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