Immune cell line with enhanced anti-tumor effect

An immune cell, tumor technology, applied in the field of tumor cell immunotherapy, can solve problems such as limitation of anti-tumor activity and T cell apoptosis

Active Publication Date: 2021-03-05
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the anti-tumor activity of the first-generation CAR-T cells is limited in vivo, and the reduction of T cell proliferation eventually leads to the apoptosis of T cells.

Method used

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  • Immune cell line with enhanced anti-tumor effect
  • Immune cell line with enhanced anti-tumor effect
  • Immune cell line with enhanced anti-tumor effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The method for constructing and verifying the cell line of the present invention comprises the following steps: step S1, site selection of RNAi

[0033] source of selection and lentiviral vectors;

[0034] Step S2, cell preparation;

[0035] Step S3, determining the drug sieve concentration;

[0036] Step S4, infection experiment of U937 cells; Step S5, drug screening;

[0037] Step S6, the expression identification of PrP;

[0038] Step S7, determination of the inhibitory effect of the cell line on tumor cells and the proliferation rate. Wherein, obtaining the construct of the cell line

[0039]Building further includes the following steps:

[0040] 1. Determine the interference sequence of the PRNP gene: GAGCAGGCCCATCATACAT. After the lentiviral vector is produced by a commercial company, the cell line is constructed according to the conventional experimental protocol.

[0041] 2. Cell preparation

[0042] (1) Resuscitating cells: Quickly take out the frozen ce...

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Abstract

The invention relates to the field of cellular immunotherapy of tumors, in particular, the invention relates to enhancing the anti-tumor effect of immune cells by modifying specific genes of the immune cells, specifically enhancing the anti-tumor effect of the immune cells by reducing the expression amount of PRNP genes (prion protein, PrP and prion protein) through technical means. The PrP is knocked down from a human tissue cell lymphoma cell U937 cell line to obtain an immune cell line with low PrP expression. The cell line has enhanced the inhibition capability on the growth of colon cancer and melanoma cells of human or mouse, and the growth level of autologous cells is increased after the immune cell line is induced by tumor cells.

Description

technical field [0001] The present invention relates to the field of tumor cell immunotherapy, in particular to enhancing the anti-tumor effect of immune cells by modifying specific genes of immune cells, specifically reducing the expression of PRNP gene by technical means to enhance the anti-tumor effect of immune cells. Background technique [0002] As one of the major diseases that threaten human health today, cancer has attracted much attention in its treatment. Among all tumor treatment methods, the efficacy of immune cell therapy has been clinically proven, and among them, Chimeric Antigen Receptor T-Cell Immunotherapy (CAR-T) is considered to be the most effective. One of the promising treatment modalities [1] . [0003] The basic principle of CAR-T action is to use immune cells to eliminate cancer cells. In a normal body, T cells can recognize and eliminate abnormal cells through the contact of antigen-presenting cells with MHC on the surface of abnormal cells; on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10A61K35/13A61K35/17A61P35/00
CPCC12N5/0635C12N5/0636C07K14/47A61K35/13A61K35/17A61P35/00
Inventor 赵宇岚张显凯
Owner EAST CHINA NORMAL UNIV
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