Method for extracting effective component in verbena and application of effective component in dysmenorrhea treatment
An active ingredient, the technology of verbena, applied in a method and its application field in the treatment of dysmenorrhea, can solve the problems of waste of medicinal value of verbena, insufficient research on verbena, and no clear understanding of the effect, etc., and achieve low price , easy to popularize and realize the effect of industrialized production and low equipment cost
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Embodiment 1
[0023] (1) Take Verbena medicinal material, remove the root, dry and chop it, pass it to 20-30 mesh, add 5 times the amount of 80% ethanol to soak for 6 hours, heat to 50 ° C, reflux extraction 3 times, extract After 1 hour, the extracts were combined, the filtrate was collected after suction filtration, and the filtrate was concentrated under reduced pressure to produce an ethanol extract of verbena equivalent to 1 g / mL;
[0024] (2) Get 1 part of the Verbena ethanol extract, extract 3 times with 1 part of petroleum ether and 1 part of chloroform successively, combine the extracts to obtain the Verbena extract, after the Verbena extract is evaporated to dryness, water Dissolved to obtain a 1g / mL verbena extract liquid;
[0025] (3) Take 40mL of 1g / mL Verbena extract medicinal solution, add 500mL of water, and decoct with slow fire for 120 minutes to make an oral solution.
[0026] Usage and dosage: twice a day, 30mL each time.
Embodiment 2
[0028] (1) Take Verbena medicinal material, remove the root, dry it and chop it, pass through a 30-mesh sieve, add 10 times the amount of 90% ethanol to soak for 12 hours, heat to 60 ° C, reflux extraction 4 times, extraction time 2 hour, combined the extracts, collected the filtrate after suction filtration, concentrated the filtrate under reduced pressure, and made the Verbena ethanol extract equivalent to 2g / mL;
[0029] (2) Get 2 parts of the Verbena ethanol extract, extract 4 times with 1 part of ethyl acetate and 2 parts of n-butanol successively, combine the extracts to obtain the Verbena extract, and evaporate the Verbena extract to dryness Finally, dissolve in water to obtain the verbena extract liquid of 2g / mL;
[0030] (3) Take 40mL of 2g / mL Verbena extract liquid, add 500mL of water, decoct with slow fire for 120 minutes, and make oral liquid.
[0031] Usage and dosage: twice a day, 30mL each time.
Embodiment 3
[0033] (1) Take Verbena medicinal material, remove the root, dry and chop it, pass it to 20-30 mesh, add 5 times the amount of 80% ethanol to soak for 6 hours, heat to 50 ° C, reflux extraction 3 times, extract After 1 hour, the extracts were combined, the filtrate was collected after suction filtration, and the filtrate was concentrated under reduced pressure to produce an ethanol extract of verbena equivalent to 1 g / mL;
[0034] (2) Get 1 part of the Verbena ethanol extract, extract 3 times with 1 part of petroleum ether and 1 part of n-butanol successively, combine the extracts to obtain the Verbena extract, and evaporate the Verbena extract to dryness , dissolved in water to obtain a 1g / mL verbena extract liquid;
[0035] (3) Take 40mL of 1g / mL Verbena extract liquid, add 100mL of water, decoct with slow fire for 120 minutes, cool to room temperature naturally, add dextrin to a total of 500g, mix, granulate, dry, pass 60-mesh sieve, whole grain, packaged, 10g per bag, mad...
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