Plant fermentation broth and preparation method and application thereof
A technology of plant fermentation broth and starter, which is applied in the directions of bacteria, applications, and plant raw materials used in food preparation, can solve the problems of poor taste and low utilization rate of medicinal materials, and can enhance human resistance and regulate intestinal microflora. Ecological, easily absorbed effect
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Embodiment 1
[0042] Screening of Bifidobacterium lactis SF-B-20 strain
[0043] (1) Source of bacteria: feces of patients with kidney deficiency
[0044] (2) Plate medium: beef extract 10.0g, yeast extract powder 5.0g, peptone 10.0g, glucose 20.0g, K 2 HPO 4 2.0g, sodium acetate 5.0g, MgSO 4 ·7H 2 O 0.2g, MnSO 4 4H 2 O 0.1g, Tween 80 1.0g, ammonium citrate 2.0g, agar 15.0g, add distilled water to 1000ml.
[0045] (3) Isolation of strains
[0046] The sample liquid was diluted tenfold, and 100 μL of each gradient was evenly spread on the MRS solid plate medium, then taken out and placed in an anaerobic tank, and incubated overnight at 37°C. On the next day, colonies of various shapes and sizes could be seen growing on the plate, and some characteristic colonies of lactic acid bacteria were picked for second-generation streak purification, and the plate was anaerobically activated for 1 day to obtain cultures, which were then identified for strain identification.
[0047] (4) Determi...
Embodiment 2
[0053] Preparation of plant fermentation broth
[0054] (1) Squeeze carrots, passion fruit, and walnuts separately for later use; crush dried rehmannia glutinosa, yam, dogwood, Alisma, Poria cocos, Moutan bark, cinnamon sticks, and aconite, and add water 1-3 cm higher than the powder Cooking to obtain extracts of various medicines;
[0055] (2) 5 parts of carrot juice prepared in step (1), 5 parts of passion fruit juice, 5 parts of walnut juice, 4 parts of dry rehmannia glutinosa extract, 2 parts of Chinese yam extract, 2 parts of cornus officinalis extract, 1.5 parts of Alisma extract, 1.5 parts of Poria cocos extract, 1.5 parts of Moutan bark extract, 0.5 parts of cassia twig extract, 0.5 part of aconite extract, 2 parts of L-arabinose, 2 parts of honey, 5 parts of resistant dextrin 2 parts, 2 parts of xylitol and 0.3 part of edible essence are mixed to obtain a mixed slurry;
[0056] (3) Transport the mixed slurry prepared in step (2) into a sterilization container, under...
Embodiment 3
[0066] Comparative Experiment of Plant Fermented Liquid Effects Prepared in Example 2 of the Present Invention
[0067] The kidney-tonifying plant fermented drink prepared in Example 2 was compared with the traditional method of boiling decoction and the marketed bifidobacterium lactis HN019 fermented drink with the same process. The nephropathy model was established by injecting puromycin aminonucleoside into the tail vein of SD mice, and the protective effect of the plant fermented drink prepared in the embodiment of the present invention and the control sample on kidney injury was studied.
[0068] Firstly, the Chinese herbal medicine is prepared according to the ratio of 4 parts of dried rehmannia glutinosa, 2 parts of Chinese yam, 2 parts of dogwood, 1.5 parts of Alisma, 1.5 parts of Poria cocos, 1.5 parts of Moutan cortex, 0.5 part of cassia twig, and 0.5 part of Pao Fuzi to obtain decoction. As a Chinese medicine group.
[0069] Secondly, the plant fermented drink obta...
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