Pathogenic bacteria electrochemical detection method based on DNA walker and nanoflower structure

Abstract

The invention discloses a pathogenic bacteria electrochemical detection method based on a DNA walker and a nanoflower structure, and belongs to the technical field of detection. A DNA walker is modified on the surface of the electrode, when a target object exists, a large number of free rolling circle amplification primers are released, formation of DNA nanoflowers is induced through a subsequentrolling circle amplification reaction, the effective area of the surface of the electrode is increased, a detection signal is amplified, and the detection sensitivity is effectively improved. And whenno target object exists in the solution, the DNA walker hydrolyzes the rolling circle amplification primer under the induction of exonuclease III, thereby reducing the background signal and wideningthe detection range of the sensor.

Description

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AI Technical Summary

Benefits of technology

The technology described by this patented allows for modification or enhanced activity at an elec-optical interface that can detect small amounts of substances (such as nucleic acids) without being affected negatively affecting their ability to perform its function effectively. This results from modifying certain parts of these molecules called DNA walks with specific sequences attached thereto. These modifications help them bind specifically to other molecular structures inside cells while still allowing some signals like those involved in gene expression to be detected accurately through fluorescent labelings placed near the elecrochromism layer.

Problems solved by technology

In this patented technical problem addressed in the patents relates to improving the accuracy and reproduciblence rates of detecting antibodysceptic staphylcytoxins (AS), particularly AS caused by Bacillustocholaceanis fragility strain B1(BFT). Current techniques like X-ray fluorescences analysis require long periods of exposure while maintaining their quality. However these techniques may result in false positives when analyzing samples containing other types of substances. There is thus a demand for more sensitive and reliable assays capable of quickly identifying AS without requiring lengthy steps and costly purifications.

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