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Efficient cultivation method of sterile explant of peach badam stem segment and explant

A cultivation method and explant technology are applied to the cultivation method of aseptic explants of peach almond stem segment and the field of explants, and can solve the problems of difficult disposal of waste liquid, improper disinfection and treatment of explants, and hazards. , to achieve the effect of improving the sterilization survival rate, shortening the disinfection time, and improving the disinfection effect.

Active Publication Date: 2021-03-02
INST OF FRUIT & TEA HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But mercuric acid is poisonous, it is not easy to buy in the market, and the waste liquid is not easy to deal with. If it is not recycled in time, it will easily cause environmental pollution. If it is not handled properly, it will also be harmful to people.
In addition, different varieties and different explants have different sensitivities to the type of disinfectant and the treatment time. The following prominent problems still exist in the rapid propagation of peach almond tissue culture: (1) Improper disinfection of explants, easy browning, resulting in survival (2) Incomplete disinfection of explants leads to high contamination rate, which affects the efficiency and output of tissue culture propagation of peach almond
This also limits its large-scale production breeding, application and promotion to a large extent.

Method used

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  • Efficient cultivation method of sterile explant of peach badam stem segment and explant
  • Efficient cultivation method of sterile explant of peach badam stem segment and explant
  • Efficient cultivation method of sterile explant of peach badam stem segment and explant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The cultivating method and explant of a kind of high-efficiency peach almond stem section aseptic explant of the present invention comprise the following steps,

[0025] S1 explant material selection, at the beginning of April to the beginning of July, randomly select eight different periods (see Table 1 for the specific selection time) in the field to select full axillary buds and semi-lignified from the Almond mother plant that grows vigorously and has no damage by diseases and insect pests. , annual shoots as explant material.

[0026] Concrete operation is, after the branch scissors that wiped with the ethanol of 75% (v / v) branches are cut off, remove branch blade with the medical scissors that the ethanol of 75% (v / v) wipes, keep the petiole of about 2mm, Wrap the branches with plastic wrap and bring them back to the laboratory, and store them in a 4°C refrigerator for later use.

[0027] S2 explant pretreatment, use disinfectant to carry out the first disinfectio...

Embodiment 2

[0036] In this embodiment, samples were taken in the field of a period randomly selected from the beginning of mid-May to the beginning of June (see Table 1 for the specific selection time), and other conditions were unchanged compared with Example 1.

Embodiment 3

[0038] The present embodiment is sampled at the time of embodiment two, and in step 3 is disinfected with 1% sodium hypochlorite solution for 15min, and disinfected with 2% sodium hypochlorite solution for 8, 10, 12, 15 and 18min respectively, compared with other conditions in embodiment one constant.

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Abstract

The invention discloses an efficient cultivation method of a sterile explant of a peach badam stem segment and the explant. The method comprises the following steps of selecting an explant material, selecting a branch which is full in axillary bud, semi-lignified and annual from a strongly growing badam stock plant without plant diseases and insect pests in the field from April to June as an explant material, pretreating the explant, disinfecting the branch for the first time by using a disinfectant, cutting the branch into small sections with 3-5 axillary buds or terminal buds reserved on each section for later use, disinfecting the explant, and disinfecting the small sections of the branch for the second time by using the disinfectant so as to remove microorganisms such as bacteria and fungi on the surface of the explant, prevent material pollution, carrying out explant inoculation, and inducing an initial bud of the explant. According to the method, the time period from the middle ten days of May to the beginning of June is defined as the explant collection time, the bacterium carrying amount of the explant is effectively reduced, and the disinfection and sterilization effects are greatly improved through combination of an explant pretreatment method, a sodium hypochlorite sterilization method, time and the like.

Description

technical field [0001] The invention relates to the technical field of explant cultivation, in particular to a method for cultivating highly efficient aseptic explants of peach almond stem segments and the explants. Background technique [0002] Almonds belong to Rosaceae and Almonds. Ordinary almonds are also known as almonds and almonds, and the trade name is "American almonds". They are also known as the four major nuts in the world together with walnuts, hazelnuts, and pistachios. Tobadan is a kind of almond rootstock variety. It was found by the Fruit Tree and Tea Research Institute of Hubei Academy of Agricultural Sciences that it can be used as the middle stock material of peaches. [0003] At present, seedlings are commonly used as rootstocks in the production of seedlings of fruit trees in my country, and the offspring are prone to separation, and the growth and resistance of rootstock seedlings are uneven. Vegetative propagation can obtain uniform rootstock seedl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/005
Inventor 艾小艳何华平王富荣龚林忠王会良刘勇朱炜袁野刘模发顾霞
Owner INST OF FRUIT & TEA HUBEI ACAD OF AGRI SCI
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