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Method for constructing rat model of sepsis with multiple organ dysfunction syndrome

A technology for dysfunction and rat model, which is applied in pharmaceutical formulations, organic active ingredients, medical preparations containing active ingredients, etc. Effects of low doses of lipopolysaccharide

Inactive Publication Date: 2021-02-26
YUNNAN SECOND PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Some scholars also use the improved "two-hit method" (blood loss + endotoxin or blood loss + CLP) to create animal models of sepsis, but this method is complicated and time-consuming, so it is rarely used

Method used

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  • Method for constructing rat model of sepsis with multiple organ dysfunction syndrome
  • Method for constructing rat model of sepsis with multiple organ dysfunction syndrome
  • Method for constructing rat model of sepsis with multiple organ dysfunction syndrome

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Embodiment 1

[0018] Experimental material used in the present embodiment: lipopolysaccharide (Sigma-L2880 (O55:B5)); Chloral hydrate (Solarbio); HITACHI-7171A automatic biochemical analyzer (Hitachi, Japan); Tissue microscopic imaging system (Sichuan Chengdu University of Science and Technology Jinpan Company); BH-Z ordinary optical microscope (OLYMPUS); surgical instruments (Shanghai Surgical Instrument Factory).

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Abstract

The invention discloses a method for constructing rat model of sepsis with multiple organ dysfunction syndrome, which comprises the following steps: preparing lipopolysaccharide to a corresponding concentration, performing lipopolysaccharide intravenous injection on a rat, pressing the rat, keeping a supine position, exposing the inner side of a hind limb upwards, lightly pulling out hair of a left hind limb, wiping with an alcohol cotton ball, and pressing groin to fill the femoral vein; after disinfection of the alcohol cotton ball, a 1 ml injector is slowly inserted into the blood vessel inparallel, medicine can be pushed after the needle tip is confirmed in the blood vessel, and after medicine pushing is finished, pressing the injection point with a dry cotton ball to stop bleeding; after hemostasis is completed, the rat is fed routinely, and the state of the rat is observed and recorded regularly. The animal model constructed by the method disclosed by the invention has low lipopolysaccharide dosage, the death rate is only 50%-70%, and the method is an ideal method for researching the sepsis with MODS model.

Description

technical field [0001] The invention relates to a method for constructing a rat model of sepsis complicated with multiple organ dysfunction syndrome, belonging to the technical field of animal model construction. Background technique [0002] Sepsis is one of the important causes of death in clinically critically ill patients. Infections from various parts of the body can induce sepsis; various pathophysiological changes can occur in sepsis, such as cell dysfunction, inflammatory response and anti-inflammatory effects. Unbalanced inflammatory response, changes in body metabolism, microcirculation disturbance, etc. If timely and effective treatment measures are not taken, excessive systemic inflammatory response or organ ischemia can lead to damage to important organs of the body, and eventually develop into multiple organ dysfunction syndrome (MODS), which endangers the life of the patient. [0003] Sepsis is a difficult problem in the ICU and even the entire medical field....

Claims

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Application Information

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IPC IPC(8): A61K31/715A01K67/027
CPCA01K67/027A01K2267/03A61K31/715
Inventor 修光辉李秀玲凌斌孙洁刘萍陈献忠邓琼芳
Owner YUNNAN SECOND PEOPLES HOSPITAL
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