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Bacterial strain for preventing and treating erysiphe cichoracearum of tobacco and a fermentation method thereof, and compounding agent for preventing and treating erysiphe cichoracearum of tobacco

A technology of tobacco powdery mildew and fermentation method, which is applied in the field of biological control, can solve the problems of excessive pesticide residues in tobacco leaves, environmental pollution, etc., and achieve the effects of reducing consumption, reducing environmental pollution, and reducing use

Active Publication Date: 2021-02-23
YUNNAN TOBACCO CORP QUJING BRANCH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The application provides a bacterial strain for preventing and treating tobacco powdery mildew and its fermentation method, and a compound agent for preventing and controlling tobacco powdery mildew, which are used to solve the problem that a large amount of chemical pesticides need to be used in the prevention and treatment of tobacco powdery mildew in the prior art, resulting in Environmental pollution, technical problems of excessive pesticide residues in tobacco leaves

Method used

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  • Bacterial strain for preventing and treating erysiphe cichoracearum of tobacco and a fermentation method thereof, and compounding agent for preventing and treating erysiphe cichoracearum of tobacco
  • Bacterial strain for preventing and treating erysiphe cichoracearum of tobacco and a fermentation method thereof, and compounding agent for preventing and treating erysiphe cichoracearum of tobacco
  • Bacterial strain for preventing and treating erysiphe cichoracearum of tobacco and a fermentation method thereof, and compounding agent for preventing and treating erysiphe cichoracearum of tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1 Bacillus amyloliquefaciens WSWFJ45

[0052] Bacillus amyloliquefaciens WSWFJ45, its deposit registration number is CGMCC No.20148. The biocontrol strains were screened from the strain bank of Yunnan Microbial Fermentation Engineering Research Center Co., Ltd.

[0053] Morphological characteristics of strain WSWFJ45: white and nearly round colonies on LB medium, the surface is wrinkled and raised in the early stage, and the surface is relatively flat in the later stage, with small protrusions, and the colonies are opaque. identified as Bacillus amyloliquefaciens.

Embodiment 2～4

[0054] Example 2-4 Preparation of Bacillus amyloliquefaciens WSWFJ45 fermentation broth samples F1-3

[0055] The preparation of bacterial strain WSWFJ45 fermented liquid comprises the following steps:

[0056] (1) Strain activation: Bacillus amyloliquefaciens WSWFJ45 stored at -80°C was activated on LB solid medium for 12 hours to obtain an activated strain.

[0057] (2) Seed solution preparation: use a sterile inoculation loop to scrape the activated bacterial strain in step (1) and inoculate it into 50ml LB liquid medium, and cultivate it for 24h at a temperature of 35°C and a rotation speed of 160rpm / min to obtain seed liquid.

[0058] (3) Fermentation liquid preparation: according to the ratio of bacterial species and culture liquid volume ratio of about 1:120, insert the seed liquid obtained in step (2) into the fermentation material culture medium of the workshop, at a temperature of 35°C (can be floated up and down) 0.5°C), the rotation speed is 120rpm / min and cultiv...

Embodiment 5

[0065] Embodiment 5 configures compound agent sample P1

[0066] Compound sample P1 includes the following components: Bacillus amyloliquefaciens WSWFJ45 fermentation broth sample F1, wetting aid Tween 20, low-toxic chemical pesticide 70% thiophanate-methyl wettable powder mixed.

[0067] The amount of wetting aid Tween 20 is one-thousandth of the mass of the compound sample P1 obtained.

[0068] Preparation method of compound agent sample P1:

[0069] 1) Dilute the fermented broth sample F1 with water 1000 times, then wetting aid Tween 20, mix well and set aside;

[0070] 2) After reducing the dosage of 70wt.% thiophanate-methyl wettable powder according to the instruction manual, the dosage is reduced by half, and diluted with water by 1000 times for later use;

[0071] 3) Transfer the solution prepared in steps 1) to 2) into a large container and stir evenly to obtain the compound agent sample P1.

[0072] The method for preparing the compound preparation from the fermen...

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Abstract

The application discloses a bacterial strain for preventing and treating preventing and treating erysiphe cichoracearum of tobacco and a fermentation method thereof, and a compounding agent for preventing and treating the erysiphe cichoracearum of the tobacco. The bacterial strain is a bacterial strain WSWFJ45; and the bacterial strain is bacillus amyloliquefaciens, and the preservation registration number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) No.20148. The bacterial strain is applied to the agricultural production process and can effectively prevent and control the erysiphe cichoracearum of the tobacco. After a bacterial strain propagation material is compounded with a medium-low toxicity chemical pesticide, the dosage of the chemical pesticide can be effectively reduced, the dosage of the pesticide is only half of the normal dosage, and the use of the low toxicity chemical pesticide can be reduced to a great extent, so that the environmental pollution is reduced, and the safety of crops is improved; and meanwhile, through detection, the prevention and treatment effect of the compounding agent is superior to that of the chemicalpesticide merely used. The bacterial strain can effectively prevent and control the erysiphe cichoracearum of the tobacco, the application amount of the chemical pesticide can be reduced to a great extent, environmental pollution is reduced and the safety of the crops is improved.

Description

technical field [0001] The application relates to a bacterial strain for preventing and treating tobacco powdery mildew, a fermentation method thereof, and a compound agent for preventing and treating tobacco powdery mildew, belonging to the technical field of biological control. Background technique [0002] Tobacco (scientific name: Nicotiana tabacum) belongs to the family Solanaceae and is an annual herb. At present, China's tobacco planting area is the largest in the world. [0003] Grass powdery mildew is commonly known as "upper gray", "lower frost" and "upper nitrate". The disease mostly damages the mature old leaves of tobacco, and the symptoms develop from bottom to top after infection. The incidence rate usually reaches 100%. When the leaves are onset, they are small yellow-brown spots that are nearly round at first, and then small white powdery spots appear on the spots, making the spots appear blanket-like, the spots expand, and the white powder covers the entir...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01N43/653A01N43/54A01N47/34A01P3/00C12R1/07
CPCC12N1/20A01N63/22A01N43/653A01N43/54A01N47/34C12R2001/07C12N1/205Y02A50/30
Inventor 蔡永占韩小女解燕何月秋孟德仁盘文政华小兵王斌莫夏明张忠全
Owner YUNNAN TOBACCO CORP QUJING BRANCH
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