Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting and separating high-purity polysaccharide from plant flowers

A high-purity, plant flower technology, applied in chemical instruments and methods, drug combinations, organic chemistry, etc., can solve the problems of low purity of polysaccharide products, injury to operators, waste of resources, etc., and achieve comprehensive utilization of resources, high-efficiency separation, remove the complete effect

Active Publication Date: 2021-02-23
HEBEI RUNSHENG AGRI TECH
View PDF8 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, this process uses a large amount of chloroform solution, which is difficult to avoid volatilization during the operation, which may cause harm to the operator; on the other hand, through the irreversible denaturation and precipitation of protein, there are more polysaccharides wrapped around, and the purity of the polysaccharide product is low, and other water-soluble substances are also ignored. Separation of components, serious waste of resources

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Weigh 50g of Xanthos sorbifolium flowers, vacuum-dry them until the water content is 15%, add 95% ethanol solution, the amount added is based on submerging the dried flower raw materials, soak twice, the soaking time is 2h, 1h respectively, and combine the soaking liquid, Vacuum filtration and separation.

[0028] (2) The filtrate was rotatably evaporated to obtain the crude flavonoid product, which was detected to be 12%, about 6 times of the flavonoid content in the raw material. Take the solid part on the filter paper and place it at room temperature. After volatilization until there is no alcohol smell, add ammonium sulfate solution with a saturation of 57%. 1.5 h, 1 h, combined extracts.

[0029] (3) The extract is sequentially passed through 001×7 type strongly acidic cation exchange resin and LX-100 type strongly basic anion exchange resin, and the down injection water is collected as crude polysaccharide aqueous solution.

[0030] (4) Add 2 times the volum...

Embodiment 2

[0032] (1) Weigh 500g of goldenflower sunflower flowers, vacuum dry to 20% moisture content, add 95% ethanol solution, the amount added is based on submerging the dried flower raw materials, soak three times, the soaking time is 2h, 1h, 1h respectively, and combine the soaking solution , separated by vacuum filtration.

[0033] (2) The filtrate was rotatably evaporated to obtain a crude flavonoid product, the content of which was detected to be 15%, which was about 5 times of the flavonoid content in the raw material. Take the solid part on the filter paper and place it at room temperature, wait for it to volatilize until there is no alcohol smell, add a sodium chloride solution with a saturation of 45% to extract polysaccharides, control the ratio of solid to liquid at 1:20, extract at a temperature of 70°C, and extract 3 times, the time is respectively 3 h, 1.5 h, 1.5 h, and combine the extracts.

[0034] (3) The extract is sequentially passed through the D296 type strongly...

Embodiment 3

[0037] (1) Weigh 500g of goldenflower sunflower flowers, vacuum dry to 20% moisture content, add 95% ethanol solution, the amount added is based on submerging the dried flower raw materials, soak three times, the soaking time is 2h, 1h, 1h respectively, and combine the soaking solution , separated by vacuum filtration.

[0038] (2) Rotary evaporation of the solvent in the filtrate to obtain the crude flavonoid product, the content of which is 15% after detection, which is about 5 times of the flavonoid content in the raw material. Take the solid part on the filter paper and place it at room temperature, wait for it to volatilize until there is no alcohol smell, add potassium nitrate solution with a saturation of 45% to extract polysaccharides, control the ratio of solid to liquid at 1:30, extract at a temperature of 85°C, extract 3 times, and the time is 3 h, 1.5 h, 1.5 h, combine the extracts.

[0039] (3) The extract is sequentially passed through 201×7 type strongly acidic...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A method for extracting and separating high-purity polysaccharides from plant flowers is disclosed and comprises the following steps: (1) drying fresh plant flowers, adding an alcoholic solution withthe concentration of more than or equal to 80%, soaking, and carrying out residue-liquid separation, (2) removing the solvent from the obtained alcoholic solution to obtain a crude flavone product, removing the solvent from the obtained residues, adding a salt solution with the saturation degree of 35-57%, and repeatedly extracting to obtain an extracting solution, (3) combining the extracting solutions, and sequentially passing through cation exchange resin, anion exchange resin and lower column water to obtain a crude polysaccharide aqueous solution, (4) adding n-butyl alcohol into the crudepolysaccharide aqueous solution for repeated extraction, combining n-butyl alcohol layer solutions and concentrating to obtain a water-soluble saponin product, combining water layers, concentrating and evaporating water, adding ethanol for precipitation, separating precipitate, and drying to obtain a polysaccharide product. According to the method, the problem of removing protein in the polysaccharide solution can be solved, crude products of medicinal components such as flavone and water-soluble saponin can be obtained through separation, and high-content polysaccharide substances can be obtained.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and relates to a method for extracting and separating high-purity polysaccharides from plant flowers. Background technique [0002] Plant polysaccharides, also known as plant polysaccharides, are polysaccharides with a degree of polymerization of more than 10 produced by plant cell metabolism. According to reports, plant polysaccharides have various medicinal physiological activities such as antioxidant, immunomodulatory and antitumor activities, and are widely used in food, medicine, and health care products, and have also become the focus of researchers in universities and institutes. [0003] The plant polysaccharides disclosed in the prior art mainly include ginseng polysaccharides, ganoderma lucidum polysaccharides, poria cocos polysaccharides, burdock polysaccharides, dendrobium flower polysaccharides and the like. The characteristics of alcohol solution, separation and preparatio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C07D311/30C07D311/40C07G3/00C07G99/00
CPCC08B37/0003C07D311/30C07D311/40C07G3/00C07G99/00
Inventor 杨香瑜杨明建昝立峰叶嘉
Owner HEBEI RUNSHENG AGRI TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com