Preparation process of supercritical particles of microbial fermentation grease

A technology for microbial fermentation and preparation process, which is applied in the field of supercritical particle preparation technology for microbially fermented oils and fats, can solve the problems of high residual oil content, high wall breaking rate, high impurity content, etc. high rate effect

Inactive Publication Date: 2021-02-19
胡勇刚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method is simple in process, but the amount of residual oil after pressing is relatively high, and the content of impurities is relatively large
Chinese patent CN106635405A discloses a method for supercritical extraction of DHA oil from microalgae powder. The method of the present invention has a high wall breaking rate, but usually has the disadvantage of low extraction yield

Method used

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  • Preparation process of supercritical particles of microbial fermentation grease
  • Preparation process of supercritical particles of microbial fermentation grease

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Effect test

Embodiment 1

[0031] The invention proposes a method for extracting oil fermented by microorganisms. The steps include: adding 250 g of microalgae powder into a supercritical reaction kettle and sealing the lid. Open CO 2 pump, making the CO 2 The fluid enters the reaction kettle, the pressure is guaranteed to be 40MPa, and the temperature is 60°C. Oil extraction is performed on the microalgae powder. After decompression separation, the extraction time is 30 minutes, and the extraction recovery rate is 86%.

Embodiment 2

[0033] The invention proposes a method for extracting oil fermented by microorganisms. The steps include: adding 250 g of microalgae powder into a supercritical reaction kettle and sealing the lid. Open CO 2 pump, making the CO 2 The fluid enters the reaction kettle, the pressure is guaranteed to be 40MPa, and the temperature is 60°C. Oil extraction is performed on the microalgae powder. After decompression separation, the extraction time is 85 minutes, and the extraction recovery rate is 98%.

Embodiment 3

[0035] The invention proposes a method for extracting oil fermented by microorganisms. The steps include: adding 250 g of microalgae powder into a supercritical reaction kettle and sealing the lid. Open CO 2 pump, making the CO 2 The fluid enters the reaction kettle, the pressure is guaranteed to be 40MPa, and the temperature is 60°C. Oil extraction is performed on the microalgae powder. After decompression separation, the extraction time is 90 minutes, and the extraction recovery rate is 98.8%.

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Abstract

The invention provides a preparation process of supercritical particles of microbial fermentation grease, and belongs to the technical field of pharmaceutical preparations. The preparation method comprises the following steps: performing grease extraction on microalgae powder in a supercritical state, and then dissolving a wall material, grease and an emulsifier in fluid CO2 to form a supercritical microemulsion; and after expanding at a certain stirring time and speed and a certain pressure and temperature, spraying to a collection kettle at a certain flow rate through an improved nozzle to form a nano-particle suspension. According to the technology, the micro-emulsion is formed in the supercritical CO2 of the medicine, and the nano-particles are collected by combining a supercritical rapid expansion technology, so that a new way is provided for the preparation of microbial fermentation grease embedding.

Description

technical field [0001] The invention relates to the field of pharmaceutical preparations, in particular to a preparation process for supercritical particles of microbial fermented oil. Background technique [0002] Docosahexaenoic acid (DHA), as a large category of omega-3 series of unsaturated fatty acids, is an indispensable fatty acid in the human body, commonly known as brain gold. However, it is difficult for the human body to synthesize itself, and most of them need to be ingested from the outside. Generally, the DHA extracted from fish oil is not high in content and contains a large amount of other unsaturated and saturated fatty acids, and DHA in fish oil has a heavy fishy smell, the content is unstable, and it is easy to oxidize. These shortcomings all restrict the development of marine fish production of DHA. [0003] Microbial oil, also known as single-cell oil (SCO), is oil-producing microorganisms such as yeast, mold, and algae that convert carbohydrates and s...

Claims

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Application Information

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IPC IPC(8): C11B1/10
CPCC11B1/104
Inventor 胡勇刚
Owner 胡勇刚
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