Hybrid broussonetia papyrifera fermented feed for ruminants and preparation method and application thereof
A technology for ruminant and fermented feed, applied in animal feed, animal feed, bacteria used in food preparation, etc., can solve the problems of low digestion and utilization rate, limited application of hybrid paper mulberry, complex protein structure, etc.
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preparation example Construction
[0090] Preparation of Lactobacillus plantarum P-8 bacterial agent
[0091] The activated Lactobacillus plantarum P-8 was inoculated into a 500ml Erlenmeyer flask equipped with 100ml of fermentation medium, cultured at 33°C for 24 hours, and the fermentation broth was collected to obtain the Lactobacillus plantarum P-8 seed solution.
[0092] Plantarum Lactobacillus P-8 seed liquid is inserted in the 500L fermenter that the fermentation medium of 200L is housed according to the inoculum size of 6% (volume ratio), at 33 ℃, cultivate 24 hours under the condition of 100rpm at stirring speed, collect All the cultured substances (fermentation liquid) in the fermenter were added with rice husk powder and dried at 50° C. to obtain Lactobacillus plantarum P-8 inoculum. The content of Lactobacillus plantarum P-8 in the Lactobacillus plantarum P-8 bacterial agent is 1×10 11 CFU / g.
[0093] Prepare per liter of fermentation medium as follows: 10g glucose, 10g peptone, 1.5g KH 2 PO 4 ,...
Embodiment 1
[0110] Example 1 Application of hybrid mulberry fermented feed for ruminants on dairy cows
[0111] 1. Preparation of hybrid mulberry fermented feed for ruminants
[0112] In this example, the raw material composition with hybrid mulberry as the main component was fermented with compound bacterial agent and / or compound enzyme to obtain three kinds of hybrid mulberry fermented feed for ruminants, which were respectively named as bacteria-enzyme synergistic fermentation of hybrid mulberry for ruminants Fermented feed, bacteria fermented alone hybrid mulberry fermented feed for ruminants and enzyme alone fermented hybrid mulberry fermented feed for ruminants. The specific preparation method is as follows:
[0113] 1. Preparation of raw material composition with hybrid tree as the main component
[0114] Weigh the following raw materials respectively: 53kg hybrid mulberry powder, 11kgDDGS, 11kg cassava residue, 15kg corn, 8kg soybean meal and 2kg molasses are mixed to obtain 100...
Embodiment 2
[0145] Example 2 Application of hybrid mulberry fermented feed for ruminants on beef cattle
[0146] The feed obtained by adding the bacteria and enzymes described in Example 1 to ferment the hybrid mulberry tree fermented feed for ruminants in the basal diet was used as a diet to feed beef cattle. The formula was adjusted according to the nutritional requirements of animals, and the reference feeding amounts of beef cattle in different physiological stages are shown in Table 4.
[0147] Table 4 The reference feeding amount of beef cattle in different physiological stages
[0148]
[0149] Select 60 crossbred cattle of 14 to 16 months old, with basically the same physical development, bred by crossbreeding Charolais cattle and local cattle, and divide them into 3 groups randomly, 20 cattle in each group. Groups II and III added 10% and 15% of their weight to the basal diet, respectively, in Table 1 in Example 1 to synergistically ferment ruminant hybrid mulberry fermented ...
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