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Ratio type quantum dot ECL aptamer sensor and manufacturing method thereof

A technology of aptamer sensor and manufacturing method, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve problems such as unsatisfactory effects, and achieve the effect of simple operation, high sensitivity, and mutual influence

Pending Publication Date: 2021-02-05
安徽理工大学环境友好材料与职业健康研究院(芜湖) +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, a series of sensing methods such as fluorescent sensing, electrochemiluminescence sensing, and chemiluminescence sensing have been developed, but the effects are not satisfactory. Therefore, a ratiometric quantum dot ECL aptasensor has been proposed in this technical field. For highly sensitive and selective detection of environmental pollutants to address the issues raised in the above background

Method used

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  • Ratio type quantum dot ECL aptamer sensor and manufacturing method thereof
  • Ratio type quantum dot ECL aptamer sensor and manufacturing method thereof
  • Ratio type quantum dot ECL aptamer sensor and manufacturing method thereof

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Embodiment 1

[0039] see Figure 1-8, the present invention provides the following technical solutions: a ratiometric quantum dot ECL aptasensor, comprising a cathode electrode and an anode electrode, the cathode electrode is a cadmium selenide quantum dot, and the anode electrode is a modified ruthenium silicon particle (RuSi@Ru( bpy)32+), the cathode electrode is connected to the anode electrode by two link chains.

[0040] Specifically, the two link chains are respectively an auxiliary chain and a capture chain.

[0041] A method for manufacturing a ratiometric quantum dot ECL aptasensor, comprising the steps of:

[0042] S1, synthesizing cadmium selenide quantum dots encapsulated by L-cysteine, comprising the following steps:

[0043] S1.1. Mix CdCl2 2.5H2O with L-cysteine ​​(the molar ratio is 1:3) and 150 mL deionized water, adjust the pH value to 10 with 0.1mol L-1 NaOH, and Carry out magnetic stirring reaction under protection to obtain cadmium (Cd) source;

[0044] S1.2. At the...

Embodiment 2

[0059] see Figure 1-8 , the present invention provides the following technical solutions: a ratiometric quantum dot ECL aptasensor, comprising a cathode electrode and an anode electrode, the cathode electrode is a cadmium selenide quantum dot, and the anode electrode is a modified ruthenium silicon particle (RuSi@Ru( bpy)32+), the cathode electrode is connected to the anode electrode by two link chains.

[0060] Specifically, the two link chains are respectively an auxiliary chain and a capture chain.

[0061] A method for manufacturing a ratiometric quantum dot ECL aptasensor, comprising the steps of:

[0062] S1, synthesizing cadmium selenide quantum dots encapsulated by L-cysteine, comprising the following steps:

[0063] S1.1. Mix 0.0913 g (0.4 mmol) CdCl2 2.5H2O with 0.1454 g (1.2 mmol) L-cysteine ​​(the molar ratio is 1:3) and 150 mL deionized water, and use 0.1 mol L- 1 NaOH to adjust the pH value to 10, and carry out magnetic stirring reaction under the protection ...

Embodiment 3

[0079] see Figure 1-8 , the present invention provides the following technical solutions: a ratiometric quantum dot ECL aptasensor, comprising a cathode electrode and an anode electrode, the cathode electrode is a cadmium selenide quantum dot, and the anode electrode is a modified ruthenium silicon particle (RuSi@Ru( bpy)32+), the cathode electrode is connected to the anode electrode by two link chains.

[0080] Specifically, the two link chains are respectively an auxiliary chain and a capture chain.

[0081] A method for manufacturing a ratiometric quantum dot ECL aptasensor, comprising the steps of:

[0082] S1, synthesizing cadmium selenide quantum dots encapsulated by L-cysteine, comprising the following steps:

[0083] S1.1. Mix CdCl2·2.5H2O with L-cysteine ​​(the molar ratio is 1:3) and 150 mL deionized water, adjust the pH value to 10 with 0.1molL-1NaOH, under the protection of nitrogen Perform magnetic stirring reaction to obtain cadmium (Cd) source;

[0084] S1....

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Abstract

The invention belongs to the technical field of new materials and analysis sensing, and particularly relates to a ratio type quantum dot ECL aptamer sensor and a manufacturing method thereof, the ratio type quantum dot ECL aptamer sensor comprises a cathode electrode and an anode electrode, the ratio type quantum dot ECL aptamer sensor is characterized in that: the cathode electrode is cadmium selenide quantum dots, and the anode electrode is modified ruthenium silicon particles (RuSi@Ru(bpy)32+); the constructed ratio type electrochemiluminescence aptamer sensor is applied to the detection ofthe antibiotic kanamycin, and has certain practical significance; ECL signals are generated by ruthenium-silicon particles and a co-reactant n-propylamine at an anode, and ECL signals are generated by cadmium selenide quantum dots and a co-reactant potassium persulfate at a cathode, so that a ratio type ECL sensor is designed, false negative existing in an experiment is eliminated, experimental data is more reliable, the selectivity of the sensor can be greatly improved by introducing an aptamer, and the sensitivity of the sensor is improved. The sensor can be applied to biological analysis or ion detection, and the designed sensor is applied to antibiotic detection of environmental analysis pollutants.

Description

technical field [0001] The invention belongs to the technical field of new materials and analysis and sensing, and can be applied to high-sensitivity and high-selectivity detection of environmental pollutants, in particular to a ratio-type quantum dot ECL aptamer sensor and a manufacturing method thereof. Background technique [0002] Many inorganic metal complexes and cluster molecules show the necessary electrochemical and optical properties as light emitters, including Ag, Al, Au, Cd, Cr, Cu, Eu, Hg, Ir, Mo, W, Os , Pd, Pt, Re, Ru, Si, Tb, Tl, etc. Especially the bipyridyl ruthenium complex Ru(bpy)32+ and its derivatives have high photochemical and electrochemical properties such as high luminous efficiency, good stability in water or non-aqueous solvents at room temperature, and reversible single-electron transfer processes at low potentials. Advantages, this type of complex is the most widely studied in the electrochemiluminescence system. [0003] The widespread prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N27/30
CPCG01N21/76G01N27/308
Inventor 石建军张馨怡
Owner 安徽理工大学环境友好材料与职业健康研究院(芜湖)
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