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Tyrosinase inhibitor and application

A tyrosinase and inhibitor technology, applied in the field of enzyme inhibitors, can solve the problems of reducing nutritional value and food quality, economic loss, color and appearance browning, etc., and achieve good inhibition of tyrosinase activity and anti-oxidation. Effect, high safety effect

Inactive Publication Date: 2021-02-05
青海瑞肽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, tyrosinase also plays an important role in the fresh-keeping industry. It is the main enzyme that causes enzymatic browning in the process of food processing and storage. Especially in the micro-processing of some vegetables and fruits, excessive melanin secretion causes The browning of color and appearance not only reduces the nutritional value and food quality, but also causes economic losses

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation method of tyrosinase inhibitor of the present invention is as follows:

[0027] (1) Enzymolysis

[0028] Take by weighing 100g of yak skin, add the Na that mass concentration is 5% 2 CO 3 Degrease and remove impurities with 5% NaCl solution, wash with pure water, incubate and pre-denature in 85-95°C aqueous solution for 2-4 hours, cool to 45-50°C, adjust pH to 8-9, add compound enzyme Enzymolysis solution Enzymolysis 4 ~ 6h. After enzymatic hydrolysis, high temperature inactivates the enzyme and cools down to room temperature. After cooling, centrifuge at 10,000 rpm for 20 minutes, collect the supernatant, and freeze-dry to obtain the lyophilized powder of the enzyme hydrolyzate.

[0029] (2) Purification

[0030] Dissolve the lyophilized powder of the enzymatic solution with ultrapure water, then separate and purify it with an ultrafiltration membrane with a molecular weight cut-off of 3000Da, freeze-dry the components below 3000Da obtained by ultr...

Embodiment 2

[0032] The tyrosinase inhibitor S of the present invention detects the activity of inhibiting tyrosinase.

[0033] Reagents: Mushroom tyrosinase (T3824-25KU) and levodopa (D9628-5G) are both products of sigma, other reagents sodium dihydrogen phosphate and disodium hydrogen phosphate are products of Sinopharm Shanghai Chemical Reagent Company.

[0034] Tyrosinase is dissolved in 0.2M phosphate buffer (pH=6.8) to prepare 100U / mL, levodopa is dissolved in 0.2M phosphate buffer and configured to 0.003M, and the tyrosinase inhibitor of the present invention is prepared with 0.2M phosphoric acid The buffer solution was dissolved and configured into different concentration solutions of 1, 2, 4, 8, and 16 mg / mL, and the enzymatic reaction was carried out according to the reaction system in Table 1 to detect its inhibitory effect on tyrosinase. Add PBS, tyrosinase and the tyrosinase inhibitor of the present invention into tubes A, B, C and D respectively according to the volume in Tab...

Embodiment 3

[0040] When the tyrosinase inhibitor of the present invention is used in food natural color-protecting agents, it has the effect of inhibiting the browning of fresh-cut fruits and vegetables.

[0041] Take 1 g of the tyrosinase inhibitor of the present invention and 0.5 g of vitamin C respectively, and dissolve them in 1000 ml of pure water to prepare a color protection solution. Take fresh, no mechanical damage and disease-free potatoes, wash them, peel them, cut them into French fries, and dry them quickly. Soak the French fries in the color protection solution for 10 minutes and then take them out and dry them. Compared with those without soaking, Store at room temperature for 24 hours to prevent browning, and store in a sealed container at -20°C to protect French fries from browning.

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Abstract

The invention discloses a tyrosinase inhibitor. The tyrosinase inhibitor is prepared by the following method of (1) enzymolysis: degreasing and removing impurities from yak by-products, then adding acompound enzyme enzymatic hydrolysate for enzymolysis, deactivating enzyme after enzymolysis is finished, cooling to room temperature, centrifuging, collecting supernatant, and freeze-drying to obtainenzymatic hydrolysate freeze-dried powder; and (2) purification: dissolving the enzymatic hydrolysate freeze-dried powder with ultrapure water, carrying out separation and purification by using an ultrafiltration membrane with the molecular weight cutoff of 3000 Da, and freeze-drying components with the molecular weight cutoff of 3000 Da or below obtained by ultrafiltration to obtain yak collagenpeptide, namely the tyrosinase inhibitor. The tyrosinase inhibitor disclosed by the invention has a relatively good tyrosinase inhibition effect, is green, environment-friendly, non-toxic and stablein activity, and has a good application prospect in the fields of beauty products and color fixatives.

Description

technical field [0001] The invention relates to an enzyme inhibitor, in particular to a tyrosinase inhibitor and its application. Background technique [0002] Tyrosinase is an oxidase, the rate-limiting enzyme that regulates melanin production in the body, and the control of its activity can change the synthesis and production of melanin. In the beauty industry, tyrosinase inhibitors are prepared into various beauty products and health foods for preventing skin tanning, whitening, and freckle removal, and are widely used in whitening effects. However, tyrosinase also plays an important role in the fresh-keeping industry. It is the main enzyme that causes enzymatic browning in the process of food processing and storage. Especially in the micro-processing of some vegetables and fruits, excessive melanin secretion causes The browning of color and appearance not only reduces the nutritional value and food quality, but also causes economic losses. Therefore, in recent years, m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K1/34C12P21/06A23L5/41A61K8/65A61Q19/02
CPCC07K14/78C12P21/06A23L5/41A61K8/65A61Q19/02A23V2002/00A61K2800/782A23V2200/048A23V2250/55A23V2250/708
Inventor 黄彪雷绍南
Owner 青海瑞肽生物科技有限公司
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