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Method for inducing cluster bud multiplication and plant regeneration of asparagus cochinchinensis

A technology of asparagus and plants, applied in the biological field, can solve the problems of low multiplication efficiency of cluster buds and difficulty in explant induction, so as to achieve the effect of improving induction efficiency and reducing production cost

Inactive Publication Date: 2021-01-29
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the object of the present invention is to provide a method for inducing asparagus cluster bud proliferation and plant regeneration, to overcome the problems of explant induction difficulties and low cluster bud proliferation efficiency in the prior art

Method used

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  • Method for inducing cluster bud multiplication and plant regeneration of asparagus cochinchinensis
  • Method for inducing cluster bud multiplication and plant regeneration of asparagus cochinchinensis
  • Method for inducing cluster bud multiplication and plant regeneration of asparagus cochinchinensis

Examples

Experimental program
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Effect test

Embodiment 1

[0024] (1) Seed treatment and germination into seedlings

[0025] The plant material Asparagus cochinchinensis (Lour.) Merr. seeds in this example comes from the Asparagus cochinchinensis (Lour.) Merr. planting base in Wuchuan County, Guizhou Province. Select mature and plump asparagus seeds, soak them in 98% concentrated sulfuric acid for 4 minutes, soak them in warm water at 35°C for 4 hours, place them on a clean bench, soak them in 75% alcohol for 0.5-1 minutes, then soak them in 0.1% mercury chloride for 10-12 minutes, Rinse with sterile water 3-5 times for later use; inoculate the seeds on the seedling medium, and induce germination and seedling growth under the conditions of temperature 25±2°C, light intensity 2500-3000lx, and light time 12h / d; The germination and seedling medium is MS+GA 3 0.5 mg / L+6-BA 1.0mg / L+KT 0.1mg / L+sucrose 30g / L+agar 7g / L, pH5.8-6.0; after 15 days of cultivation, the seeds begin to show white, and after 36 days of cultivation, the sprouts grow ...

Embodiment 2

[0035] (1) Seed treatment and germination into seedlings

[0036]The plant material Asparagus cochinchinensis (Lour.) Merr. seeds in this example comes from the Asparagus cochinchinensis (Lour.) Merr. planting base in Wuchuan County, Guizhou Province. Select mature and plump asparagus seeds, soak them in 98% concentrated sulfuric acid for 4 minutes, soak them in warm water at 35°C for 4 hours, place them on a clean bench, soak them in 75% alcohol for 0.5-1 minutes, then soak them in 0.1% mercury chloride for 10-12 minutes, Rinse with sterile water 3-5 times for later use; inoculate the seeds on the seedling medium, and induce germination and seedling growth under the conditions of temperature 25±2°C, light intensity 2500-3000lx, and light time 12h / d; The germination medium is MS+GA 3 1.0 mg / L+6-BA 1.5mg / L+KT 0.1mg / L+sucrose 30g / L+agar 7g / L, pH5.8-6.0; after 15 days of cultivation, the seeds begin to show white, and after 36 days of cultivation, the sprouts grow to more than 3...

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Abstract

The invention discloses a method for inducing cluster bud multiplication and plant regeneration of asparagus cochinchinensis. Stems, with axillary buds, of aseptic germinated seedlings of seeds are used as explants for axillary bud induction, cluster bud multiplication, rooting culture and acclimatization and transplantation to obtain plant regeneration of the asparagus cochinchinensis. By the adoption of the method, the axillary bud induction rate can reach 85.05%, the cluster bud multiplication multiple reaches 3.65, the rooting rate reaches 78.27%, the transplanting survival rate reaches 87.10%, the production cost can be effectively reduced, the tissue culture and rapid propagation period can be effectively shortened, and the method can be applied to large-scale seedling propagation and resource protection of the asparagus cochinchinensis.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for inducing asparagus cluster bud proliferation and plant regeneration. [0002] technical background [0003] Asparagus cochinchinensis (Lour.) Merr. is a perennial climbing herb of the Liliaceae Asparagus genus, also known as Asparagus, Asparagus, Dadangmengen, etc. Asparagus cochinchinensis (Lour.) Merr. Used as medicine, it has the effects of nourishing yin and moistening dryness, clearing the lungs and reducing fire. It is mainly used to treat dry-heat cough, yin deficiency and cough, fever and fluid injury, internal heat and thirst, intestinal dryness and constipation, and sore throat. Asparagus has a long history of medicinal use in my country, and it has medicinal, edible, and ornamental values, and its development and utilization have broad prospects. [0004] In recent years, due to people's unrestrained and indiscriminate digging, wild resources of as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张明生李林李扬杨宁线高燕燕杭烨
Owner GUIZHOU UNIV
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