Industrial production method for obtaining chlorella protein peptide by acid enzyme method

Abstract

The invention relates to an industrial production method for obtaining chlorella protein peptide by an acid enzyme method. The industrial production method comprises the following steps: (1) performing swelling treatment on chlorella powder to obtain chlorella feed liquid; (2) performing acid hydrolysis: adding hydrochloric acid into the chlorella feed liquid obtained after swelling in the step (1) for hydrolysis; (3) performing enzymolysis: adjusting the pH value of the chlorella feed liquid subjected to the acid hydrolysis to 7-9, then sequentially adding cellulase, alkaline protease, papainand neutral protease at the temperature of 45-60 DEG C, performing enzymolysis for 4-8 hours, and filtering out chlorella residues in enzymatic hydrolysate after enzyme deactivation, so as to obtainsupernatant; (4) decolorizing the supernatant to obtain a decolorized solution; and (5) filtering the decolorized solution obtained in the step (4) to obtain a chlorella protein peptide solution, andperforming spray drying on the chlorella protein peptide solution to obtain the chlorella protein peptide. The acid hydrolysis and biological enzyme hydrolysis are combined, the advantages are complementary, and the enzymolysis efficiency and the protein peptide extraction rate of chlorella can be improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an industrial production method for obtaining chlorella protein peptides by an acid enzymatic method. Background technique [0002] Chlorella (Chlorella spp.) is a type of single-celled green algae, known as "pharmaceutical algae". The cell shape of Chlorella is oval or oval, with a diameter between two microns and twelve microns. It is easy to cultivate, has a wide ecological distribution, and has a fast growth rate. It is a high-quality single-cell protein source, and its protein content is as high as 50-65%. , can be used as a high-protein food for the human body to quickly supplement nutrition and restore physical fitness. The amino acid composition of chlorella is higher than the protein standard for human nutrition promulgated by the World Health Organization (WHO) and the United Nations Food and Agriculture Organization (FAO), so it is listed by FAO as a healthy food for huma...

AI Technical Summary

Problems solved by technology

[0008] At present, there are many laboratory studies on chlorella active peptides, but the industrial utilization of chlorella active peptides is less

At present, the industrial research on chlorella mainly focuses on single-enzyme or compound enzyme hydrolysis (for example, the preparation method of chlorella anti-tumor polypeptide disclosed in the Chinese invention patent application specification with publication number CN102851345A, and the Chinese invention patent with publication number CN104120160A A preparation method of chlorella antioxidant peptide disclosed in the application specification), or add a second enzyme after hydrolyzing with an enzyme for a period of time (for example, a Chinese invention patent application specification with publication number CN 109402204 A disclosed A preparation method of a chlorella immunoactive peptide, the publication number is CN 109207544 A Chinese invention patent application specification discloses a preparation method of a chlorella antioxidant polypeptide), but because the chlorella cell wall is thick and rich in fiber The wall breaking rate of pretreatments such as swelling and homogenization is low, so the effect of biological enzymatic hydrolysis is poor, and the extraction rate of protein peptides is low