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Application of OsMAPKK4 gene in improving disease resistance of rice

A gene and rice technology, applied in the application field of OsMAPKK4 gene, can solve the problems of easy loss of resistance, non-durability, and narrow resistance spectrum

Active Publication Date: 2021-01-26
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the R gene is more widely used in production, but the resistance mediated by the R gene is not long-lasting except for a narrow resistance spectrum, and the resistance is easily lost; compared with the R gene, the disease resistance-related genes have a wider resistance spectrum. wide, and the resistance is not easy to lose, etc.

Method used

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  • Application of OsMAPKK4 gene in improving disease resistance of rice
  • Application of OsMAPKK4 gene in improving disease resistance of rice
  • Application of OsMAPKK4 gene in improving disease resistance of rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Analysis of the expression pattern of the OsMAPKK4 gene after rice was treated with bacterial blight and jasmonic acid

[0029] In order to verify whether the OsMAPKK4 gene is involved in the regulation of disease resistance in rice. The applicant’s research on the rice variety Zhonghua 11 (or ZH11, a model variety used for scientific research by the Institute of Crop Science, Chinese Academy of Agricultural Sciences) and MKbZH1 (a material containing the main disease resistance gene Xa26 in the background of rice variety Zhonghua 11) at the booting stage , donated by the research group of Professor Wang Shiping of Huazhong Agricultural University, a scientific research exchange material) were inoculated with bacterial blight respectively, and at the same time, the rice variety Zhonghua 11 at the tillering stage was treated with jasmonic acid, and then the OsMAPKK4 gene was analyzed by qRT-PCR expression mode. The results showed that the OsMAPKK4 gene could ...

Embodiment 2

[0030] Embodiment 2: Obtaining of OsMAPKK4 gene overexpression material

[0031] (1) Construction of OsMAPKK4 gene overexpression vector

[0032] This example is a general description about the construction of pU1301-OsMAPKK4 vector.

[0033] Using the cDNA of rice variety Zhonghua 11 as a template, primers KK4-F-KpnI (5'-GGGGTACCATGCGACCGGGCGGGCC-3') and KK4-R-KpnI (5'- GGGGTACCTCATGACGGAGGCGGTGCGA-3') were designed and used for high-fidelity DNA polymerase PCR The full-length cDNA fragment of the OsMAPKK4 gene was amplified (the 108th to 1217th deoxyribonucleotides in the sequence shown in SEQ ID NO: 1, a total of 1110 deoxyribonucleotides). The PCR product was recovered by electrophoresis, the PCR product was digested with the restriction endonuclease KpnI, the digested product was recovered by electrophoresis, and the vector pU1301 was digested overnight with KpnI and recovered. Ligate the recovered enzyme-digested product with the carrier fragment at a molar ratio of abou...

Embodiment 3

[0038] Example 3: Correlation Analysis and Functional Verification of OsMAPKK4 Gene Overexpression Rice Lines

[0039] (1) Phenotype analysis of resistance to bacterial blight at booting stage of rice lines overexpressed with OsMAPKK4 gene

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Abstract

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of an OsMAPKK4 gene in improving disease resistance of rice, particularly the application in controlling resistance of rice to bacterial blight and bacterial leaf streak. Through research on overexpression of the OsMAPKK4 gene, it is found that the OsMAPKK4 gene can influence the resistance of rice to bacterial leaf blight and bacterial leaf streak, and the overexpression of the OsMAPKK4 gene improves the resistance of rice to bacterial leaf blight and bacterial leaf streak. The OsMAPKK4 gene can be applied to disease-resistant breeding of rice.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and specifically relates to the application of the OsMAPKK4 gene. The gene is the rice OsMAPKK4 gene, and the gene can be used to enhance the cultivation of rice varieties with broad-spectrum resistance to bacterial blight and bacterial streak disease. Background technique [0002] The disease resistance genes in rice can be mainly divided into two categories: main disease resistance genes and disease resistance related genes. Among them, the main disease resistance genes are also called R genes, which are usually only resistant to one or a few pathogenic races. Resistance genes, such as cloned Xa21, xa13, Xa3 / 26, Xa1 / 2 / 14, etc.; disease resistance genes other than the main disease resistance genes are called disease resistance-related genes, such as OsWRKY45, OsWRKY13, OsEDR1, OsPAD4, etc. At present, the R gene is more widely used in production, but the resistance mediated by ...

Claims

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Application Information

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IPC IPC(8): C12N15/54C12N15/82A01H5/00A01H6/46
CPCC12N9/12C12N15/8281C12Y207/11
Inventor 袁猛陈洁杨泽宇
Owner HUAZHONG AGRI UNIV
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