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Sterile treatment agent for macromolecular protein and/or nucleic acid as well as preparation method and application of sterile treatment agent

A treatment agent and macromolecular technology, which is applied in the field of aseptic treatment agent for macromolecular protein and/or nucleic acid and its preparation, can solve the problems of heat intolerance, short shelf life, blockage, etc., to improve safety, Strong bactericidal effect

Inactive Publication Date: 2021-01-22
SHANGHAI CHUANG HONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such substances are not tolerant to heat, and high temperature and high pressure sterilization methods cause irreversible damage to the substance itself
Pasteurization can sterilize milk, which is a huge improvement, but it is only used for food, and it does not have a long shelf life, and it does not meet the requirements for medical use
Sterile filtration is also a commonly used method, but for large molecules, it is difficult to effectively pass through the filter holes, resulting in huge component loss and blockage

Method used

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  • Sterile treatment agent for macromolecular protein and/or nucleic acid as well as preparation method and application of sterile treatment agent
  • Sterile treatment agent for macromolecular protein and/or nucleic acid as well as preparation method and application of sterile treatment agent
  • Sterile treatment agent for macromolecular protein and/or nucleic acid as well as preparation method and application of sterile treatment agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] This embodiment provides a kind of aseptic treatment agent for macromolecular protein and / or nucleic acid, and its preparation method comprises the following steps:

[0023] S1. Accurately weigh 0.2 g of 5,5-dimethylhydantoin, 0.2 g of Tween-80, 0.5 g of human α-defensin 5, and 10 g of phosphate buffer solution, and set aside. Among them, the pH of the phosphate buffer solution is 7.2, which is obtained by dissolving 1.546 g of disodium hydrogen phosphate, 0.262 g of sodium dihydrogen phosphate, and 8.768 g of sodium chloride with water and diluting to 1000 mL.

[0024] S2. At room temperature, sequentially add 5,5-dimethylhydantoin, Tween-80, and human α-defensin 5 into the phosphate buffer solution weighed above, and stir with a magnetic stirrer at 500 rpm Stir and mix for 30 minutes at a divided speed to obtain a uniform and stable aseptic treatment agent.

Embodiment 2

[0026] This embodiment provides a kind of aseptic treatment agent for macromolecular protein and / or nucleic acid, and its preparation method comprises the following steps:

[0027] S1. Accurately weigh 1.5g of 5,5-dimethylhydantoin, 1.5g of Tween-80, 5g of human α-defensin 5, and 3g of phosphate buffered saline, and set aside. Among them, the pH of the phosphate buffer solution is 7.2, which is obtained by dissolving 1.546 g of disodium hydrogen phosphate, 0.262 g of sodium dihydrogen phosphate, and 8.768 g of sodium chloride with water and diluting to 1000 mL.

[0028] S2. At room temperature, sequentially add 5,5-dimethylhydantoin, Tween-80, and human α-defensin 5 into the above-mentioned weighed phosphate buffer solution, and use a magnetic stirrer at 1500 rpm Stir and mix for 30 minutes at a divided speed to obtain a uniform and stable aseptic treatment agent.

Embodiment 3

[0030] This embodiment provides a kind of aseptic treatment agent for macromolecular protein and / or nucleic acid, and its preparation method comprises the following steps:

[0031] S1. Accurately weigh 0.3g of 5,5-dimethylhydantoin, 0.3g of Tween-80, 4g of human α-defensin 5, and 9g of phosphate buffer solution, and set aside. Among them, the pH of the phosphate buffer solution is 7.2, which is obtained by dissolving 1.546 g of disodium hydrogen phosphate, 0.262 g of sodium dihydrogen phosphate, and 8.768 g of sodium chloride with water and diluting to 1000 mL.

[0032] S2. At room temperature, sequentially add 5,5-dimethylhydantoin, Tween-80, and human α-defensin 5 into the above-mentioned weighed phosphate buffer solution, and stir with a magnetic stirrer at 800 rpm Stir and mix for 30 minutes at a divided speed to obtain a uniform and stable aseptic treatment agent.

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Abstract

The invention is applicable to the technical field of disinfectants, and provides a sterile treatment agent for macromolecular protein and / or nucleic acid as well as a preparation method and application of the sterile treatment agent. The sterile treatment agent comprises the following components in parts by weight: 0.2-1.5 parts of 5, 5-dimethyl hydantoin, 0.2-1.5 parts of Tween-80, 0.5-5 part ofhuman alpha defensin 5 and 3-10 parts of a buffer solution. The sterile treatment agent can thoroughly kill bacteria in a macromolecular protein and / or nucleic acid solution, does not destroy proteins and nucleic acids, and does not increase heat source substances in the solution while killing bacteria.

Description

technical field [0001] The invention belongs to the technical field of disinfectants, and in particular relates to a sterile treatment agent for macromolecular proteins and / or nucleic acids, a preparation method and application thereof. Background technique [0002] Sterile processing of proteins and nucleic acids has always been a difficult problem. Such substances are not tolerant to heat, and the high temperature and high pressure sterilization method will cause irreversible damage to the substance itself. Pasteurization can sterilize milk, which is a huge improvement, but it is only used for food, and it does not have a long shelf life and does not meet the requirements for medical use. Sterile filtration is also a commonly used method, but for large molecules, it is difficult to effectively pass through the filter holes, resulting in huge component loss and blockage. [0003] More and more macromolecular proteins and nucleic acids have been developed. If a treatment a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/50A01N37/46A01P1/00A01P3/00A01P13/00
CPCA01N37/46A01N43/50
Inventor 高俊锋张秉金沈明志赖志
Owner SHANGHAI CHUANG HONG BIOTECH
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