LAMP primers and kit for efficient triple detection of three pathogens of Chinese softshell turtle

Abstract

The invention discloses LAMP primers and a kit for efficient triple detection of three pathogens of Chinese softshell turtle. The kit comprises a reaction system for microfluidic chip detection. The reaction system for microfluidic chip detection comprises the primer for detecting TSHSV, the primer for detecting TSFV, the primer for detecting SYJ15, and a reaction solution. The primer for detecting the TSHSV, the primer for detecting the TSFV and the primer for detecting the SYJ15 are respectively pre-buried in a microfluidic chip. According to the method, the TSHSV, the TSFV and the SYJ15 canbe rapidly and accurately detected at the same time.

Description

technical field [0001] The invention relates to the technical field of pathogen detection, in particular to a LAMP primer and a kit for efficient triple detection of three pathogens of Chinese soft-shelled turtle. Background technique [0002] Chinese soft-shelled turtle (Trionyx sinensis), commonly known as soft-shelled turtle, water fish or turtle, belongs to Reptilia (Reprilia), Chelonia (Chelonia), turtle family (Trionychidae), and turtle genus (Triomx)") in taxonomy, and is mainly distributed in China. , Russia, Japan, Vietnam and other Southeast Asian countries and regions, mostly inhabit freshwater waters with gentle currents such as rivers, lakes, ponds and reservoirs, where fish and shrimp proliferate. The research on soft-shelled turtle disease in China started relatively late, in the early 1980s Almost no relevant research work has been carried out. With the rapid development of intensive soft-shelled turtle breeding, the incidence and mortality of soft-shelled tu...

AI Technical Summary

Problems solved by technology

At present, the technology used in the molecular diagnosis of pathogens is mainly PCR technology, but because the reaction needs to cycle in two different temperature zones, the requirements for the instrument are high, and the cost is relatively high, and the PCR technology only has one pair of amplification primers, which is susceptible to interference. The specificity is relatively insufficient, and the result takes a long time, the operation professional requirements are high, and there are many micro-dosing steps

Moreover, it takes a long time to detect multiple pathogenic factors one by one, the operation is cumbersome, and reagents are wasted

The LAMP technology developed in recent years has 4 different specific primers, so the detection result is more accurate, but because it is a constant temperature reaction, it lacks a hot-start enzyme similar to PCR, and it is prone to non-specific amplification during the heating stage of the equipment, which affects Test results

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