2D and 3D cell co-culture system capable of implementing continuous harvesting without enzyme digestion and construction method and application thereof

Abstract

The invention discloses a 2D and 3D cell co-culture system capable of implementing continuous harvesting without enzyme digestion and a construction method and application thereof. The construction method of the 2D and 3D cell co-culture system comprises: (1) preparing a seal with a microarray pattern; (2) preparing a culture substrate with a microarray pattern; (3) co-culturing 2D and 3D cells onthe micro-pattern culture substrate; and (4) obtaining a 3D cell microsphere and 2D cell co-culture system. According to the cell 2D and 3D co-culture system capable of implementing continuous harvesting without enzyme digestion, which is provided by the invention, 2D and 3D coexsiting co-culture of the cells can be implemented in the same environment, and if 3D cell microspheres need to be obtained, the 3D cell microspheres can be harvested by simple blowing and beating through a simple suction pipe without enzyme digestion; In addition, the substrate cells can still continue to generate the3D cell microspheres just like that continuous harvesting can be implemented by planting plants, meanwhile, collagen, sodium alginate and other materials do not need to be added in the formation of the 3D cell microspheres, so as to reduce the influence of exogenous extracellular matrixes in research.

Description

technical field [0001] The invention belongs to the technical field of cell co-culture systems, and in particular relates to a 2D and 3D cell co-culture system that can be harvested continuously and does not require enzyme digestion, and its construction method and application. Background technique [0002] Cell culture is an important experimental technique in biological and medical research, and plays an irreplaceable role in basic research, drug evaluation and other experiments. However, there are still some problems to be solved urgently in cell culture technology. [0003] 1. 2D culture conditions cannot reflect the real 3D culture environment of cells. [0004] 2. The currently commonly used 3D cell culture system needs to add gel or solid components such as collagen and sodium alginate as a scaffold. If the choice is not good, the material composition will be very different from the real cell environment. [0005] 3. There are also some specific cells (such as tumor...

AI Technical Summary

Problems solved by technology

However, there are still some problems to be solved in cell culture technology

[0003] 1. 2D culture conditions cannot reflect the real 3D culture environment of cells

[0004] 2. The currently commonly used 3D cell culture system needs to add collagen, sodium alginate and other gel or solid components as scaffolds. If the choice is not good, the composition of the material is very different from the real cell environment

[0005] 3. There are also some specific cells (such as tumor cells), which can form microspheres in suspension culture due to their strong self-renewal ability, but the microspheres are freely suspended in the culture medium and cannot be fixed, which affects fixed-point and regular observation.

[0006] 4. At present, compared with 2D culture, 3D cell culture has complicated technology and needs to be prepared on demand each time, which cannot achieve sustainable acquisition

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