Fluorescence detection probe and method for determining drug residues in poultry liver tissues

A technology for fluorescence detection and liver tissue, applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of no active recognition sites, increase, and affect recognition performance, so as to improve specific selectivity and reduce viscosity. Attachment, increase the effect of solubility

Inactive Publication Date: 2021-01-12
郑程程
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the anti-interference ability of the tetracycline carbon dot fluorescent molecular imprinting material is not good, and it is easy to bind and adsorb with coexisting interference substances such as biological macromolecular proteins in the detection system, which affects its recognition performance; at the same time, the material also has specific selectivity. Poor, active recognition sites not greatly improved and increased technical flaws

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Preparation of fluorescent detection probes:

[0048] P1, Preparation of nitrogen-doped fluorescent carbon dots: using biomass materials as precursors, nitrogen-doped fluorescent carbon dots were prepared by hydrothermal synthesis. The specific process was: washing and crushing biomass waste such as dragon fruit peels Slurry, weigh 10g and disperse in 20mL ethanol solvent, after ultrasonic treatment for 30min, transfer to hydrothermal reaction kettle, hydrothermal reaction at a constant temperature of 200°C for 10h, after the reaction, the reaction kettle is naturally cooled to room temperature, and the obtained light brown The solution was ultrafiltered with a filter membrane to remove large particle aggregates, and dialyzed for 12 hours to prepare a CDs-N solution, which was processed by freeze-drying to prepare nitrogen-doped fluorescent carbon dots;

[0049] P2, preparation of fluorescent microspheres: disperse 5 mmol of oxytetracycline in 1.5 L of hydrochloric a...

Embodiment 2

[0074] The difference from Example 1 is that during the preparation of the fluorescent detection probe, the amount of oxytetracycline added in step P2 is 1 mmol.

Embodiment 3

[0076] The difference from Example 1 is that in the preparation process of the fluorescent detection probe, the amount of oxytetracycline added in step P2 is 10 mmol.

[0077] Compared with Example 1, in the fluorescent detection probe-oxytetracycline composite systems prepared in Example 2 and Example 3, the detection limits of oxytetracycline were 26.8 ng / mL and 24.5 ng / mL, respectively. The above data indicate that the addition amount of the template agent oxytetracycline can affect the detection ability of the fluorescent detection probe.

[0078] In summary, the present invention provides a fluorescent detection probe and a method for determining drug residues in poultry liver tissue. In the present invention, nitrogen-doped fluorescent carbon dots are used as fluorescent materials, oxytetracycline is used as a template, and methacrylic acid and 2,3-propylene oxide methacrylate are used as polymerized monomers. The above-mentioned copolymerization product is prepared by hy...

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Abstract

The invention provides a fluorescence detection probe and a method for determining drug residues in poultry liver tissues. According to the invention, nitrogen-doped fluorescent carbon dots are takenas a fluorescent material, oxytetracycline is taken as a template agent, methacrylic acid and 2, 3-epoxypropane methacrylate are taken as polymerization monomers, a copolymerization product is obtained through a polymerization reaction, and then hydrophilic modification treatment is carried out on the copolymerization product to obtain a product. According to the method for determining the drug residue in the poultry liver tissue, the self-made fluorescence detection probe is adopted for determining the drug residue, the method is simple and rapid to operate, the detection time is greatly shortened, the complex separation process in a traditional method can be effectively avoided, and the selective recognition capacity of oxytetracycline can be efficiently improved; and the sensitivity andthe selectivity of the fluorescence detection probe are effectively improved.

Description

technical field [0001] The invention relates to the technical field of drug residue detection, in particular to a fluorescent detection probe and a method for measuring drug residues in poultry liver tissue. Background technique [0002] Tetracycline antibiotics are broad-spectrum antibiotics, which are widely used in the field of veterinary medicine and widely used in animal feed because of their good oral absorption capacity, convenient use, low toxicity and low cost, and broad-spectrum antibacterial activity. However, the residual tetracycline in poultry animal tissue has certain safety hazards: eating this animal tissue product may cause allergic reactions in humans and lead to drug resistance of various bacteria, which seriously threatens human health. [0003] Due to their good water solubility, optical stability, excellent biocompatibility, low biological toxicity, and strong fluorescence properties, fluorescent carbon dots have shown good application prospects in the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/65G01N21/64C08F220/20C08F220/06C08F220/32C01B32/15
CPCC08F220/20C09K11/025C09K11/65C01B32/15G01N21/6428G01N2021/6432C08F220/06C08F220/32
Inventor 郑程程
Owner 郑程程
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