Sequencing library building instrument capable of continuously feeding samples and operation method thereof

Abstract

The invention provides a sequencing library building instrument capable of continuously feeding samples and an operation method thereof. The sequencing library building instrument at least comprises aworking space, wherein a continuous sample feeding module, a TIP head module, a reagent storage module and at least two PCR modules are arranged on the bottom side of the working space; a liquid transferring module and a magnetic rod sleeve module are movably arranged in the working space; the liquid transferring module moves in a space where the continuous sample feeding module, the TIP head module, the reagent storage module and the at least two PCR modules are located in a reciprocating manner; the magnetic rod sleeve module at least moves in a space where the PCR modules are located in areciprocating manner; the continuous sample feeding module at least comprises a plurality of rows of sample feeding reaction tubes which are arrayed at intervals; the magnetic rod sleeve module comprises a magnetic rod assembly and a magnetic rod sleeve assembly; the magnetic rod assembly and the magnetic rod sleeve assembly are matched to move to realize adsorption and release of magnetic beads;and continuous sample feeding of a plurality of samples can be realized and the utilization of consumable items is reduced.

Description

technical field [0001] The invention relates to the field of gene sequencing library construction, in particular to a sequencing library construction instrument for continuous sampling and an operation method thereof. Background technique [0002] The rapid acquisition of gene sequence information is very important for life science research. At present, the most commonly used method is nucleic acid high-throughput gene sequencing, which can sequence hundreds of thousands to hundreds of millions of DNA molecules at one time. In order to make the DNA extracted from living organisms meet the requirements of sequencing, it is necessary to perform end repair, adapter addition, purification and other operations on the interrupted DNA. This experimental process is called library construction. Connect the desired adapters at both ends. The library structure must meet the requirements of the high-throughput sequencer before sequencing. The process of library construction is divided i...

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Problems solved by technology

[0003] Although the current automatic sequencing library construction instrument can also complete the library construction of nucleic acid samples, there are several fatal problems: 1. It can only realize the sample loading of 16 channels at most. If more samples are required to be loaded For detection, it is necessary to replace the consumables and configuration parameters in the library construction instrument. It takes 2-3 hours to reconfigure the sequencing library construction instrument each time, resulting in a long time for library construction, and it is difficult to meet the mixed library construction of multiple samples; 2. The cost of consumables High, it takes a lot of TIP heads to complete a sample loading and building a library, and the transfer process of samples and magnetic beads is complicated and easy to cause cross-contamination

3. At present, the library construction instruments on the market include PCR instruments, resulting in high cost and difficulty in later maintenance

4. Most of the library construction is only completed to the PCR library purification process, and the library dilution and mixing before loading the machine are not included.

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