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Application of anaphalis virgata n-butyl alcohol extract

A kind of green n-butanol and extract technology, which is applied in the field of biological activity of traditional Chinese medicine plants, can solve the problems such as no report on the biological activity research of A.

Active Publication Date: 2021-01-01
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, there is no report on the biological activity research of Xingqing

Method used

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  • Application of anaphalis virgata n-butyl alcohol extract
  • Application of anaphalis virgata n-butyl alcohol extract
  • Application of anaphalis virgata n-butyl alcohol extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] a. Take 30g of Cinnamomum fruticosa, after pulverization, use 70% ethanol to heat and reflux in a water bath with a mass-volume ratio of 20 times, the temperature of the water bath is 60°C, extract twice for 1.5 hours each time, and collect the extract;

[0025] b. Distill the extract under reduced pressure to remove the ethanol therein, concentrate to syrup, and dry at a temperature of 45°C to obtain the crude extract of Cymbidium sinensis;

[0026] c. After dispersing the extract of the crude extract of Panzhixiangqing with 1:15-1:20 in water, it is sequentially extracted with petroleum ether, dichloromethane, ethyl acetate and n-butanol, and the n-butanol The extract was evaporated under reduced pressure to a syrupy state, and dried at 45°C to obtain the n-butanol extract of Cymbidium chinensis.

Embodiment 2

[0028] The n-butanol extract of the phoenix radix that embodiment 1 obtains is used for biological activity screening:

[0029]Using reduced nicotinamide adenine dinucleotide phosphate (NADPH), DL-glyceraldehyde mixture as a substrate, and the positive drug quercetin as a control, high-throughput screening of aldose reductase was performed using a microplate reader , according to the presence of quantitative and excess nicotinamide adenine dinucleotide phosphate (NADPH), using DL-glyceraldehyde as a substrate to detect the reduction of nicotinamide adenine dinucleotide phosphate (NADPH) to reflect aldose Reductase (Aldose Reductase, AR) activity, the composition of the enzyme reaction system is as follows, phosphate buffered saline solution (pH7.4), aldose reductase, nicotinamide adenine dinucleotide phosphate (NADPH), DL-glyceraldehyde, Quercetin (3.125 μg / mL), a specific inhibitor of aldose reductase, mixed the reaction system and placed it in the dark for 30 minutes, and me...

Embodiment 3

[0035] The n-butanol extract obtained in Example 1 affects the mouse kidney podocytes (MPC-5) viability: the mouse kidney podocytes are inoculated in a 96-well plate, 8000 / well, and cultivated for 16 hours to make After the cells adhered to the wall, the medium was replaced to establish a high glucose injury model. The control group was cultured in 10% fetal bovine serum + 100U / mL penicillin + 100U / mL streptomycin + 11mmol / L D-glucose 1640 medium for 24 hours, and the model ( High glucose) group was stimulated with 10% fetal bovine serum + 100U / mL penicillin + 100U / mL streptomycin + 30mmol / L D-glucose 1640 medium for 24 hours; The cyanobutanol extract was cultured for 24 hours, and after 24 hours, the cell viability was detected by 3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide salt colorimetric method (MTT) ; Use Graphpad Prism statistical analysis to test the statistical difference between the control group and the administration group. The research shows that: a...

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Abstract

The invention relates to application of an anaphalis virgata n-butyl alcohol extract. The anaphalis virgata n-butyl alcohol extract is obtained from anaphalis virgata through 70% ethanol thermal extraction, and in-vitro cell experiments show that the anaphalis virgata n-butyl alcohol extract has the effects of reducing blood sugar and relieving kidney lesions caused by high glucose injury. According to the extract, the glucose consumption of rat myoblasts (L6) and the Na <+> - K <+> - ATP enzymatic activity of a high-glucose induced high-glucose injured mouse kidney cell (MPC-5) model can be remarkably increased, and the effects of reducing blood sugar and regulating a polyol pathway to relieve kidney lesions caused by high-glucose injury are shown.

Description

technical field [0001] The invention belongs to the field of biological activities of traditional Chinese medicinal plants, and relates to the use of the n-butanol extract of Cymbidium sinensis, in particular to the preparation of medicines for treating diabetes and diabetic nephropathy caused by high-sugar damage. Or the use in preventing diabetes and assisting hypoglycemic health care products. It has been confirmed by cell experiments that it has the effect of lowering blood sugar and improving diabetic nephropathy caused by high sugar damage. Background technique [0002] Anaphalis virgata, a plant of the genus Anaphalis virgata in the family Asteraceae, grows at an altitude of 3000-4000 meters, mainly in western Tibet, western Xinjiang, Central Asia, Iran and other regions. The genus Cymbidium is composed of 80 species of plants, mainly distributed in tropical and subtropical regions of Asia; at present, about 50 species have been found in China, mainly concentrated in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P3/10A61P13/12A23L33/105
CPCA23V2002/00A61K36/28A61K2236/333A61K2236/39A61K2236/55A23L33/105A61P3/10A61P13/12A23V2200/328
Inventor 信学雷胡苗马依努尔·拜克力诺莫诺夫·索迪克买迪娜阿吉艾克拜尔·艾萨
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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