Efficient screening method for parents required by preparation of fast-growing larimichthys polyactis new strain
A screening method, the technology of small yellow croaker, is applied in the field of high-efficiency screening of parents required for the preparation of new strains of fast-growing small yellow croaker, which can solve the problems of low accuracy and achieve the effect of improving accuracy
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Embodiment 1
[0019] (1) Family construction
[0020] Collect about 4,000 small yellow croakers from the artificial breeding group, and raise them in the same cage until December (the group reaches about 8 months of age). Randomly select 200 small yellow croakers, measure their body weight, and sort them to determine the body weight value of the top 30%. As a standard, the remaining 3,800 small yellow croakers were measured, and the individuals with the top 30% of the body weight were selected as the first-generation parents. Live clamworms were fed to strengthen parental cultivation, and the water temperature was adjusted to maintain at 15°C to promote gonad development.
[0021] (2) PIT marker of small yellow croaker with high survival rate
[0022] At the beginning of April of the following year, male and female parents with excellent gonad development were selected. According to the mating method of 1 male parent and 1-2 female parents, 30 small yellow croaker families were constructed ...
Embodiment 2
[0039] Experiment with the method of Example 1, collect about 5200 small yellow croakers in the artificial culture group, and breed them in the same net cage until December (the group reaches about 8 months old), randomly take 200 fish, measure their body weight, and sort them to determine the size before sorting. 30% of the body weight value, using this as a standard, measure the remaining 5,000 small yellow croakers, and select the individuals whose body weight is in the top 30%, and finally obtain 589 small yellow croakers. Strengthen cultivation, control the water temperature and keep it at 16°C to promote gonad development. 34 small yellow croaker families were obtained by artificial oxytocin fertilization and hatching. All the families were raised separately in 2500L barrels, and the seedlings were regularly separated according to the power requirements. At the age of 5 months, the average body weight of each family was sampled and measured. 200 tails were mixed with PIT...
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