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Preparation method of spectral near-infrared electrochemical luminescence immunosensor

A chemiluminescence immunological and immunosensor technology, applied in the field of analysis, to achieve the effects of simple operation, rich content and detection index information, and high light intensity

Active Publication Date: 2020-12-29
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Up to now, there is no report on spectral ECL immunoassay method or method with a radiation band exceeding 850 nanometers

Method used

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  • Preparation method of spectral near-infrared electrochemical luminescence immunosensor
  • Preparation method of spectral near-infrared electrochemical luminescence immunosensor
  • Preparation method of spectral near-infrared electrochemical luminescence immunosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] All the utensils were soaked in freshly prepared aqua regia for 24 hours, rinsed thoroughly with ultrapure water and ethanol, and then dried in the air; 0.4mM methionine, 0.25mL Au solution (96mM) and 1.2mL Ag solution ( 10mM) was dissolved in 4ml of ultrapure water and ultrasonically mixed, then 1M sodium hydroxide was added to adjust the pH value to 12.0, and incubated at 37°C for 10 hours. Centrifuge the prepared solution at 5000 rpm to remove large particles at the bottom, add 0.5 ml of 1M sulfuric acid solution to the supernatant, centrifuge and precipitate the initial gold nanoparticles at 8000 rpm, remove the supernatant, and add the final centrifuged precipitate to 3mL of 2% ammonia water, incubate in an oven at 80°C for 20 minutes, and finally centrifuge the prepared quantum dots with isopropanol at 13300 rpm, and store them in a refrigerator at 4°C. The fixed concentration of the bimetallic nanocluster solution is 1mg / mL Test the fluorescence spectrum, such as...

Embodiment 2

[0086] The steps are the same as in Example 1, except that the test method for the bimetallic nanocluster solution is an ultraviolet spectrogram, such as figure 2shown.

Embodiment 3

[0088] The steps are the same as in Example 1, except that the prepared bimetallic nanocluster solution is dropped on a copper grid to observe the morphology. The high-magnification transmission electron microscope photos of the gold-silver bimetallic nanoclusters made by the test, such as image 3 shown.

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Abstract

The invention belongs to the field of analysis technical methods, and relates to preparation of a spectral near-infrared electrochemical luminescence (915nm) immunosensor. The preparation method mainly comprises the following steps that (1), methionine is used as a stabilizer to prepare a water-soluble Au-Ag bimetallic nanocluster capable of generating near-infrared electrochemical luminescence; (2), a gold-silver bimetallic nanocluster-marked secondary antibody (Au-AgAb2) is prepared; (3), a spectral near-infrared electrochemical luminescence sensor is prepared by taking the gold-silver bimetallic nano gold-silver cluster as a marker on the basis of a sandwich immunity principle; and (4), a working curve is drawn. The spectral electrochemical luminescence immunosensor is high in detectionsensitivity, and other antigen proteins do not interfere with the sensing detection of the target antigen.

Description

technical field [0001] The invention belongs to the field of analytical techniques and methods, and relates to a preparation method of a spectrum-type near-infrared electrochemiluminescent immunosensor using gold-silver bimetallic nanomaterials as markers. Background technique [0002] At present, most electrochemiluminescence (ECL) biochemical analysis and clinical diagnosis use ruthenium bipyridine whose maximum radiation wavelength is in the red light region (620 nm) as a marker, and it is realized by detecting the total intensity of ECL radiation. The development of new ECL biochemical analysis techniques and detection methods with different radiation bands is of great significance to promote the progress of ECL technology. For example: Chinese patent document CN104764737B discloses a light-intensity ECL immunoassay method with a radiation band located in the green light region (550nm) and with CdSe quantum dots as a marker; Chinese patent document CN102749452A discloses...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N27/48G01N27/327G01N33/574
CPCG01N21/76G01N27/3278G01N27/48G01N33/57473G01N33/57476G01N33/57484
Inventor 邹桂征傅莉
Owner SHANDONG UNIV
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