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Bacillus megatherium and application of fermentation liquor of bacillus megatherium to promoting plant germination

A technology of Bacillus megaterium and fermentation broth, which is applied in the application field of Bacillus megaterium and its fermentation broth in promoting plant germination, can solve problems such as relatively rare reports of growth promoting and antagonistic properties, and achieve the effect of promoting germination

Active Publication Date: 2020-12-29
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, in the phosphate-solubilizing bacteria agents such as Bacillus megaterium studied so far, only the phosphorus-solubilizing ability of the strain has been paid attention to, and there are relatively few reports on its growth-promoting and antagonistic properties.

Method used

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  • Bacillus megatherium and application of fermentation liquor of bacillus megatherium to promoting plant germination
  • Bacillus megatherium and application of fermentation liquor of bacillus megatherium to promoting plant germination
  • Bacillus megatherium and application of fermentation liquor of bacillus megatherium to promoting plant germination

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Embodiment 1

[0031] This embodiment provides a kind of Bacillus megaterium JL-1 producing phytase ability assay method, wherein, the isolated strain Dq-1 adopted in the test is Bacillus megaterium JL-1 (Bacillus megaterium JL-1) in It was deposited in the China Center for Type Culture Collection on November 13, 2019, the address is Wuhan University, China, and the preservation number is CCTCC NO: M 2019928.

[0032] In addition, the method for determining the ability to produce phytase is as follows:

[0033] Centrifuge the Bacillus megaterium JL-1 fermentation broth at 6000r / min for 5min and dilute appropriately, then take 0.4mL and add 1mL of 5mmol / L sodium phytate solution prepared with pH 4.4 acetic acid buffer, keep it warm at 37°C for 30min, and finally add color Terminate mixed solution 1.0mL, 700nm determination of inorganic phosphorus content. For the control, 1.0 mL of color termination mixture was added first, then 0.4 mL of centrifuged diluted fermentation broth was added, and...

Embodiment 2

[0037] This embodiment provides an experimental method for measuring the ability of Bacillus megaterium to produce auxin (indole acetic acid, IAA) and siderophore, which includes the following steps:

[0038] (1) Determination of IAA production ability: Take 1 mL of fermentation liquid and inoculate it into King's liquid medium for 24 hours before testing, take 2 mL of bacterial liquid and centrifuge at 12,000 r / min for 3 min, take 1 mL of supernatant in a test tube, add 2 mL of Sakowski reagent at room temperature in the dark The reaction was carried out for 30 minutes, and the absorbance was measured at 530 nm after the reaction, and the uninoculated King's liquid medium was used as a blank control.

[0039] IAA output = n × (A i -A 0 -0.0213) / 0.0308.

[0040](2) Determination of the siderophilic ability of the strain: After the test strain was shaken and cultivated in the MKB medium for 48 hours, the fermentation broth was centrifuged for 5 minutes at a speed of 10000 r / m...

Embodiment 3

[0054] This embodiment provides a method for preparing a fermentation broth of Bacillus megaterium JL-1 as follows: take the Bacillus megaterium JL-1 bacterial classification in the slant culture medium and inoculate it into 50mL LB medium, and inoculate it in a shaker at 31°C After 24 hours of shaking culture, the fermentation broth of Bacillus megaterium JL-1 was obtained; each liter of the LB medium included 6g of yeast powder, 12g of sodium chloride, and 12g of peptone, the pH was adjusted to 7.0, 0.1MPa, and sterilized at 121°C for 20min.

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Abstract

The invention is applicable to the technical field of microorganisms, and provides bacillus megatherium and an application of fermentation liquor of the bacillus megatherium to promoting plant germination. The bacillus megatherium and the fermentation liquor thereof not only have strong phosphate solubilizing capacity, but also have the capacity of generating auxin and siderophore, and the potential growth promoting and antagonism functions of a strain are shown. The bacillus megatherium can be used for promoting germination of mung beans and other plants. Specifically, after the bacillus megaterium and the fermentation liquor thereof provided by the invention are properly diluted, the activity of a stress-resistant enzyme in seed germination can be improved, and the malondialdehyde content is reduced.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to the application of a bacillus megaterium and its fermented liquid in promoting plant germination. Background technique [0002] Phosphorus is one of the three major nutrients necessary for plant growth. Phosphorus deficiency has an impact on plant photosynthesis, respiration and biosynthesis. At present, the total phosphorus content in different types of soil in my country is 0.31-1.72g·Kg -1 , the content of available phosphorus is 0.1~228.8mg·Kg -1 , the available phosphorus accounts for less than 5% of the soil phosphorus content, which is far from meeting the needs of crop growth and development, and must be supplemented by the application of phosphorus fertilizers. At present, the utilization rate of phosphorus fertilizers in my country is only 10-25%. Most of the phosphate ions will be fixed, causing soil compaction and a decline in fertility. At the sam...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01N43/38A01P21/00C12R1/11
CPCC12N1/20A01N63/22A01N43/38C12R2001/11C12N1/205
Inventor 林志伟肖翠红孙冬梅
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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