Methods, uses and kits for monitoring or predicting response to periodontal disease treatment

Abstract

Disclosed is an in vitro method for assessing or predicting the response of a human patient to treatment of periodontal disease. The method is based on the insight to determine biomarker proteins. Accordingly, in a sample of saliva of a patient, the concentrations are measured of certain protein combinations. One such combination is Hemoglobin subunit delta (Hb-delta) and Pyruvate kinase (PK). Another such combination is Keratin-4 (K-4) and at least two of Alpha-1-acid glycoprotein (A1AGP), Pyruvate Kinase (PK) and Matrix metalloproteinase-8 (MMP-8). A third combination is Alpha-1-acid glycoprotein (A1AGP), Pyruvate Kinase (PK) and S100 calcium binding protein A8 (S100A8). Based on the concentrations as measured, at least one value is determined reflecting the joint concentrations for saidproteins. This at least one value may indicate the probability that human patient has been or will be successfully treated for the periodontitis. The at least one value can be compared with at leastone threshold value reflecting in the same manner the joint concentrations associated with successful treatment of periodontitis. The comparison allows assessing whether the testing value is indicative of the periodontal treatment status in said patient.

Description

technical field [0001] The present invention is in the field of oral care and relates to saliva based assessment of response to periodontal disease treatment. In particular, the present invention relates to a kit, use and method for assessing or predicting response to treatment of a patient suffering from periodontal disease. Background technique [0002] Inflammation of the gums or gingivitis is a non-destructive periodontal disease mainly caused by the adhesion of dental bacterial biofilm or plaque to the tooth surface. If undetected and treated, reversible gingivitis typically triggers inflammation of the tissue surrounding the tooth (the periodontal tissue), a condition defined as periodontitis, which is irreversible and leads to tissue destruction and alveolar bone Missing, eventually leading to the loss of teeth. As gum disease develops, there are often clinical signs and symptoms associated with it, such as gum swelling, changing color from pink to dark red, bleedin...

AI Technical Summary

Problems solved by technology

However, the available methods for performing this assessment involve a labor-intensive process that makes it impossible for the dentist to perform routinely on each patient and / or visit, and which is impossible to perform by the user (self-diagnosis)

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