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Composite microbial agent for degrading kitchen waste and preparation method thereof

A technology of composite bacterial agent and kitchen waste, applied in the field of environmental protection, can solve the problems of high preparation cost, complex bacterial agent components, limited degradation efficiency, etc., and achieve the effect of high production efficiency and simple preparation process

Pending Publication Date: 2020-12-18
深圳市嘉佳环保技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently reported bacterial agents for food waste reduction treatment have relatively complex components, high preparation costs, and limited degradation efficiency.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022]The preparation method of the composite bacterial agent for degrading kitchen waste provided in this embodiment includes the following steps:

[0023](1) Bacillus subtilis, Bacillus licheniformis and Bacillus coagulans were respectively inoculated into beef extract peptone medium and cultured at 30°C with shaking for 60 hours, wherein the formula of the beef extract peptone medium was: beef extract 5g / L , Peptone 5g / L, sodium chloride 0.5g / L, dipotassium hydrogen phosphate 1g / L;

[0024](2) Separately inoculate Candida lyticus and plant lactic acid bacteria in potato dextrose culture medium, and shake culture at 30°C for 72 hours. The formula of the potato dextrose culture medium is: potato powder 10g / L, glucose 20g / L;

[0025](3) Weigh the following activated strains in parts by weight: 15 parts of Bacillus subtilis, 20 parts of Bacillus licheniformis, 20 parts of Bacillus coagulans, 8 parts of plant lactic acid bacteria, and 12 parts of Candida unculis. The weighed activated strains...

Embodiment 2

[0028]The preparation method of the composite bacterial agent for degrading kitchen waste provided in this embodiment includes the following steps:

[0029](1) Bacillus subtilis, Bacillus licheniformis and Bacillus coagulans were respectively inoculated into beef extract peptone culture medium and cultured at 30°C with shaking for 72 hours, wherein the formula of the beef extract peptone culture medium was: beef extract 5g / L , Peptone 5g / L, sodium chloride 0.5g / L, dipotassium hydrogen phosphate 1g / L;

[0030](2) Separately inoculate Candida lyticus and plant lactic acid bacteria in potato dextrose culture medium, and shake culture at 30°C for 72 hours. The formula of the potato dextrose culture medium is: potato powder 10g / L, glucose 20g / L;

[0031](3) Weigh the following activated strains in parts by weight: 20 parts of Bacillus subtilis, 25 parts of Bacillus licheniformis, 20 parts of Bacillus coagulans, 5 parts of plant lactic acid bacteria, and 15 parts of Candida lyticus. The weighed a...

Embodiment 3

[0034]The preparation method of the composite bacterial agent for degrading kitchen waste provided in this embodiment includes the following steps:

[0035](1) Bacillus subtilis, Bacillus licheniformis and Bacillus coagulans were respectively inoculated into beef extract peptone medium, and cultured at 30°C with shaking for 48 to 72 hours, wherein the formula of the beef extract peptone medium is: beef extract 5g / L, peptone 5g / L, sodium chloride 0.5g / L, dipotassium hydrogen phosphate 1g / L;

[0036](2) Separately inoculate Candida lyticus and plant lactic acid bacteria in potato dextrose culture medium, and shake culture at 30°C for 48-72h, wherein the formula of the potato dextrose culture medium is: potato powder 10g / L, glucose 20g / L;

[0037](3) Weigh the following activated strains in parts by weight: 18 parts of Bacillus subtilis, 24 parts of Bacillus licheniformis, 22 parts of Bacillus coagulans, 10 parts of plant lactic acid bacteria, and 15 parts of Candida lyticus. The weighed activ...

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Abstract

The invention discloses a composite microbial agent for degrading kitchen waste and a preparation method thereof. The preparation method comprises the following steps: respectively inoculating bacillus subtilis, bacillus licheniformis and bacillus coagulans into a beef extract peptone culture medium for culturing; respectively inoculating candida utilis and plant lactobacillus into a potato glucose culture medium for culturing; and performing mixed fermentation on the bacillus subtilis, bacillus licheniformis, bacillus coagulans, candida utilis and plant lactobacillus to prepare the compositemicrobial agent. By researching compounding of multiple microbial agents, the synergistic effect among strains in a proper proportion is discovered, and main components (cellulose, grease, starch andprotein) of the kitchen waste can be effectively degraded, so that the kitchen waste can be fully fermented. Moreover, the preparation process provided by the invention is simple, and the production benefits are high.

Description

Technical field[0001]The invention relates to the technical field of environmental protection, in particular to a composite bacterial agent for degrading kitchen waste and a preparation method thereof.Background technique[0002]Food waste is high in nutrients, so it is easy to emit foul smell, rot and deteriorate, and spread bacteria and viruses. In addition, the water in it is very easy to pollute the groundwater through osmosis, and the waste grease and residue can easily block the sewers, causing serious environmental pollution. At present, the main methods of processing food waste include: (1) Burying. Centralized burying of kitchen waste and other domestic waste is the simplest and cheapest method. However, as the scale of the city expands and the cost of land rises, look for Appropriate landfill sites have also become difficult. At the same time, this method is likely to cause groundwater pollution and the spread of infectious diseases; (2) It is directly recycled and used as f...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16B09B3/00C12R1/10C12R1/125C12R1/07C12R1/25C12R1/72
CPCC12N1/20C12N1/16B09B3/00
Inventor 黄河唐勇
Owner 深圳市嘉佳环保技术有限公司
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