Method for in-vitro cloned embryo transplantation of pregnant sow

Abstract

The invention discloses a method for in-vitro cloned embryo transplantation of a pregnant sow. The method comprises the following steps: feeding an estrus synchronization agent to each sow at a fixedpoint every day, after continuously feeding each sow with the estrus synchronization agent for 18 days, performing intramuscular injection of PG600 5 days later, and selecting an individual with normal estrus for in-vitro cloned embryo transplantation 1 day later. According to the method, a proper microenvironment is provided for the pregnant sow after the cloned embryo is transplanted by virtue of feeding or injection of the estrus synchronization agent and the PG600, synchronism of the estrus, ovulation and embryo transplantation time of the pregnant sow is controlled, and the success rate of cloned embryo implantation and the pregnancy rate of the pregnant sow are greatly increased, so that batch production of cloned pigs is realized, and a foundation is laid for large-scale productionof the cloned pigs.

Description

technical field [0001] The invention belongs to the fields of estrus induction and cloned embryo transplantation, and in particular relates to a method for in vitro cloned embryo transplantation to surrogate sows. Background technique [0002] Somatic cell cloning technology has great application potential in the expansion and reproduction of excellent livestock breeds, the protection of endangered breeds, the production of transgenic animals, animal disease models, organ transplantation and other fields. However, the cloning efficiency is still very low. Generally, the success rate of livestock is only 1% to 2% (number of surviving sons / cloned embryos). For pigs, the success rate is even lower than 1%, resulting in a huge waste of embryos and surrogate sows. The death of a large number of embryos implanted at the same time has also aroused more ethical concerns. The estrus time and ovulation time of cloned embryo transplanted surrogate sows are difficult to determine, and ...

AI Technical Summary

Problems solved by technology

However, the cloning efficiency is still very low. Generally, the success rate of livestock is only 1% to 2% (number of surviving sons / cloned embryos). For pigs, the success rate is even lower than 1%, resulting in a huge waste of embryos and surrogate sows. Simultaneous implantation of a large number of embryo deaths has also raised more ethical concerns

The estrus time and ovulation time of surrogate sows for cloned embryo transfer are difficult to determine, and the inconsistency of the uterine implantation synchronization between cloned embryos in vitro and surrogate sows and other technical problems restricts the key factors for the mass production and application of this technology

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