A method for detecting pesticide residues in Chinese medicinal materials

Abstract

The disclosure provides a method for detecting pesticide residues in Chinese herbal medicines, and relates to the field of pesticide detection. The pulverized Chinese herbal medicines to be tested are mixed with water and subjected to a first extraction to obtain a first extraction product; the first extraction product is mixed with acetic acid Acetonitrile aqueous solution is mixed and subjected to the second extraction to obtain the second extraction product; the second extraction product is mixed with anhydrous magnesium sulfate and sodium chloride and subjected to the third extraction to obtain the third extract; after the third extract is centrifuged Obtain the supernatant as the matrix extract, mix the matrix extract with the purification agent and perform purification treatment, and then centrifuge the purified material as the test solution; use ultra-high performance liquid chromatography-tandem mass spectrometry to test liquid is measured. The detection method can realize the simultaneous detection of up to 76 kinds of pesticides including insecticides, fungicides and plant growth regulators in Chinese herbal medicines, and has high accuracy and repeatability.

Description

technical field [0001] The present disclosure relates to the field of pesticide detection, in particular, to a method for detecting pesticide residues in Chinese medicinal materials. Background technique [0002] Traditional Chinese medicine is one of the most important cultural heritages in my country, and has the functions of health care, prevention and treatment of diseases. Today, with the development of modern science and technology, traditional Chinese medicine is gradually entering the world. In recent years, the planting area of ​​traditional Chinese medicine in China has grown rapidly. There are more than 430 planting bases of common varieties, and the planting area is about 3.067 million hectares. 2 . In recent years, people have paid more and more attention to the safety of traditional Chinese medicine, because traditional Chinese medicine must not only ensure its efficacy, but also ensure its safety. Some studies have pointed out that about 65-80% of people pr...

AI Technical Summary

Problems solved by technology

However, according to the national standard 2763 and the Chinese Pharmacopoeia 2015, my country has set maximum residues for about 600 kinds of pesticides in agricultural products, but among Chinese medicinal materials, only 4 kinds of Chinese medicinal materials have stipulated a total of 7 kinds of pesticides mainly organochlorine pesticides Maximum residue limits, which lead to inconsistent standards between exporting countries and importing countries in the process of exporting traditional Chinese medicine, which has become a stumbling block for Chinese medicine to go global

[0004] The matrix of traditional Chinese medicinal materials is relatively complex, and the multi-residue detection of pesticides has the defects of low accuracy and poor repeatability

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