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Nitric oxide in-situ hydrogel for treating osteoarthritis

A technology for osteoarthritis and nitric oxide, which is applied in the fields of anti-inflammatory agents, bone diseases, inorganic active ingredients, etc., can solve the problem of high-efficiency carrying of nitric oxide gas that has not been seen yet, and achieve long-term controlled release treatment , convenient storage and transportation, and convenient use

Active Publication Date: 2020-11-24
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although in situ hydrogel has many advantages in joint cavity drug delivery and local treatment, nitric oxide is a gas, and there is no research report on how to efficiently carry nitric oxide gas in in situ hydrogel

Method used

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  • Nitric oxide in-situ hydrogel for treating osteoarthritis
  • Nitric oxide in-situ hydrogel for treating osteoarthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Preparation of nitric oxide in situ hydrogel

[0025] According to the component ratios in Table 1, the nitric oxide in situ hydrogel of the experimental group was prepared, which specifically included the following steps:

[0026] a: Disperse polyoxyethylene-polyoxypropylene-polyoxyethylene copolymer and heparin-poloxamer in 6 times the mass of 8°C water for injection, place it in a refrigerator at 4-8°C overnight, dissolve slowly, and add Acidic fibroblast growth factor with equal molar mass of heparin-poloxamer, mixed to form a hydrogel matrix solution;

[0027] b: Take egg yolk phospholipids and poloxamer with a mass ratio of 1:25, mix them, dissolve them in anhydrous tert-butanol 10 times the mass at 65°C, slowly cool down until the solution solidifies, let stand overnight at -10°C, and freeze-dry to obtain The loose freeze-dried powder is transferred into a stoppered bottle, filled with nitric oxide gas to saturation, added to water for injection with 5...

Embodiment 2

[0033] Example 2 Application Effect of Nitric Oxide In Situ Hydrogel for Treating Osteoarthritis

[0034] (1) Establishment of model animals

[0035] Taking the Diannan small-ear pig as the research object, 20% papain (papain) was injected into the right knee joint of the adult Diannan small-ear pig. After 4 weeks, the articular cartilage degeneration, osteoblast activity, and osteophyte formation were evaluated by histology. , confirm that the modeling is successful.

[0036] (2) The application effect of nitric oxide in situ hydrogel in the treatment of osteoarthritis

[0037] The adult Diannan mini-ear pigs with right knee osteoarthritis successfully modeled were selected and divided into several groups according to the design in Table 1. They were injected with 2 mL of nitric oxide in situ hydrogel into the right knee joint cavity, reared routinely, and sacrificed after 2 months. , through histological staining, the pathophysiological conditions of the cartilage were obs...

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Abstract

The invention provides a nitric oxide in-situ hydrogel for treating osteoarthritis. The nitric oxide in-situ hydrogel comprises a hydrogel matrix and nitric oxide nanobubbles, wherein the nitric oxidenanobubbles are uniformly dispersed into the hydrogel matrix. The hydrogel matrix is composed of a catechole-terminated polyoxyethylene-polyoxyepropylene-polyoxyethylene copolymer, heparin-poloxamerand an acidic fibroblast growth factor; the mass ratio of the catechole-terminated polyoxyethylene-polyoxyepropylene-polyoxyethylene copolymer to the heparin-poloxamer is (1-5):10; and the molar massof the acidic fibroblast growth factor is equal to that of the heparin-poloxamer. The nitric oxide nanobubbles are composed of vesicles formed by wrapping a nitric oxide gas with egg yolk lecithin andpoloxamer as bubble film materials; the mass ratio of the egg yolk lecithin to the poloxamer is 1:25; the particle size range is 500-900 nm; and the concentration is 1*10<7> / mL to 5*10<7> / mL. The nitric oxide in-situ hydrogel provided by the invention is injected into an articular cavity for treating the osteoarthritis.

Description

technical field [0001] The invention relates to an in-situ preparation for treating osteoarthritis, in particular to a nitric oxide in-situ hydrogel for treating osteoarthritis. Background technique [0002] Osteoarthritis (OA) is a common chronic progressive arthritis, and its pathological features are degeneration and destruction of articular cartilage and subchondral bone sclerosis. Reactive hyperplasia of joint margins and subchondral bone, osteophyte formation. Osteoarthritis is more common in the elderly. Epidemiological surveys show that its incidence rate is 40% among people aged 55-64, and 60% among people over 65 years old. With the acceleration of the aging of the world population and the With the prosperity of sports, the incidence of osteoarthritis is also increasing year by year. Osteoarthritis is a chronic degenerative disease characterized by cartilage degeneration accompanied by synovial tissue inflammation. Osteoarthritis patients suffer from pain and jo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K47/22A61K47/36A61K47/24A61K38/18A61P19/02A61P19/08A61P29/00A61K33/00
CPCA61K9/0024A61K9/06A61K47/22A61K47/36A61K47/24A61K33/00A61K38/1825A61P19/02A61P19/08A61P29/00A61K2300/00
Inventor 赵应征鲁翠涛徐荷林姚情陈睿兰清华
Owner WENZHOU MEDICAL UNIV
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