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Quantitative method for turtle egg laying amount and fertilization rate based on PIT markers and application

A fertilization rate and marking technology, which is applied in the quantitative field of turtle egg production and fertilization rate, can solve the problems of wear, tortoise shell shedding, lettering or edge punching individual effective marking, etc., to reduce manpower, material and financial resources, and recycle The effect of high rate, high research value and scientific significance

Active Publication Date: 2020-11-24
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The inventor of the present application has used methods such as engraving on the tortoise shell or punching holes on the edge of the tortoise shell, but because the tortoise shell will fall off naturally, and the daily activities of the turtle, such as climbing and fighting each other, will cause the edge of the tortoise shell to wear. Therefore, Although this method can distinguish individuals in a short period of time, it can be as short as half a year or as long as a year, and neither lettering nor edge punching can effectively mark individuals

Method used

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  • Quantitative method for turtle egg laying amount and fertilization rate based on PIT markers and application
  • Quantitative method for turtle egg laying amount and fertilization rate based on PIT markers and application
  • Quantitative method for turtle egg laying amount and fertilization rate based on PIT markers and application

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Embodiment 1

[0035] The present invention firstly discloses a method for quantifying the amount of eggs laid and the fertilization rate of turtles based on PIT markers. The method flow chart is shown in figure 1 shown. The specific method is as follows:

[0036] (1) Use PIT electronic tags to mark female turtles.

[0037] Before the breeding season (from the end of March to the beginning of April), when the weather is fine and the temperature is suitable, inject the PIT electronic tag into the abdominal cavity of the female turtle along the gap between the carapace and the plastron to accommodate the hind limbs (such as figure 2shown). Disinfect the electronic tag with potassium permanganate before injection, and then use 75% alcohol cotton ball to disinfect the peritoneum where the needle is inserted. About 2.5-3cm. After the injection, use a cotton swab dipped in erythromycin ointment and apply evenly to the needle site to prevent wound inflammation. Finally, use a handheld scanner...

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PUM

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Abstract

The invention belongs to the technical field of biological breeding, and particularly relates to a quantitative method for turtle egg laying amount and fertilization rate based on PIT markers and application. According to the invention, the PIT mark is injected into the abdominal cavity through the gap part for accommodating the hind limb between the dorsal turtle and the ventral turtle, so as tomark the turtles. A hanging label method is applied, namely, the PIT numbers are registered on the manufactured round paper labels through marking pens, nylon ropes are tied to the tail ends of the bamboo sticks, the bamboo sticks are inserted beside spawning points of female turtles when spawning of the turtles is finished and burying of eggs is about to be completed, and the turtle eggs are collected the next morning. The PIT marker is combined with a tag hanging method, so that the problem that the egg laying amount and the fertilization rate of individuals are unknown can be solved.

Description

technical field [0001] The invention belongs to the technical field of biological breeding, and in particular relates to a quantitative method and application of PIT marker-based turtle egg production and fertilization rate. Background technique [0002] PIT (passive integrated transponder) tag, also known as passive integrated transponder, is a method for tracking and identifying animals based on radio frequency identification tags. It has been widely used in animal behavior, growth, and resource monitoring in recent years. PIT markers are widely used in fish, but limited in other aquatic animals, especially in turtles with thick and hard exoskeleton on the back. [0003] Turtles are nocturnal egg-laying animals, and they do not have egg-protecting behavior after the egg-laying is over. Therefore, under breeding conditions, it is difficult for the existing marking technology to accurately correspond the nesting point of the turtle with the egg-laying female parent. On the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02Y02A40/81
Inventor 王亚坤朱新平刘晓莉于凌云袁菊李伟洪孝友谢敏敏
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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