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A method and application of rapid ammonia nitrogen removal by non-sterile fermentation of microalgae in extreme environment

An extreme environment and fermentation technology, applied in the field of rapid ammonia nitrogen removal, can solve the problems of high efficiency, low-cost treatment and high-value resource utilization of unfavorable wastewater, and achieve more guaranteed biological safety of wastewater, lower costs and higher efficiency Effect

Active Publication Date: 2021-08-10
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Therefore, the prior art has not yet organically combined the use of microalgae fermentation to quickly remove ultra-high concentrations of ammonia nitrogen and co-produce high-value phycobiliprotein biomass in non-sterile wastewater systems, which is not conducive to the realization of high-efficiency, Low-cost processing and high-value resource utilization

Method used

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  • A method and application of rapid ammonia nitrogen removal by non-sterile fermentation of microalgae in extreme environment
  • A method and application of rapid ammonia nitrogen removal by non-sterile fermentation of microalgae in extreme environment
  • A method and application of rapid ammonia nitrogen removal by non-sterile fermentation of microalgae in extreme environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Example 1 Adaptive cultivation of ultra-high ammonia-nitrogen industrial waste water to thiophilic Prototheca

[0090] 1.1 Activation of algal species

[0091] Single algae colonies of thiaphophilic primordial red algae (Galdieria sulphuraria UTEX 2919) were picked out from the solid medium and inoculated in the 2MA medium after adjusting the formula for cultivation. The pH value of the medium was 2.5, and the glucose concentration was 20g / L. At a culture temperature of 35 °C and a light intensity of 210 μmol m -2 the s -1 , cultured in a constant temperature light shaker with a rotational speed of 150 rpm (cultivated to the logarithmic growth phase). Wherein, the formula of 2MA medium after formula adjustment is shown in Table 1.

[0092] 2MA medium formula after adjusting the formula in table 1

[0093]

[0094]

[0095] Note: EDTA is ethylenediaminetetraacetic acid; in the original 2MA medium formula (NH 4 ) 2 SO 4 The concentration is 2.62g / L.

[0096]...

Embodiment 2

[0103] Example 2 The effect of non-sterile culture system on the ammonia nitrogen removal efficiency of thiaphilic red algae

[0104] 2.1 Activation of algal species and preparation of wastewater medium

[0105] The preparation of the activated red algae (Galdieria sulphuraria UTEX 2919) and the non-sterile ultra-high ammonia nitrogen wastewater medium were carried out according to the methods described in 1.1 and 1.2, respectively.

[0106] 2.2 Non-sterile culture system for the cultivation of thiaphilic Rhodophyta

[0107] 2.2.1 Mixed cultivation: Inoculate the algae into a conical flask containing 100mL (volume 250mL) non-sterile ultra-high ammonia nitrogen wastewater medium, where the initial ammonia nitrogen concentration is 2800mg / L, and non-inoculated red algae The sterilized wastewater medium was used as the blank group. The inoculation density of red algae in the culture system was 1×10 8 cells / ml, the pH value of the medium is 2.5, and the glucose concentration is...

Embodiment 3

[0110] Example 3 Continuous treatment of ultra-high ammonia-nitrogen industrial wastewater by thiophilic red algae in a fermenter culture system

[0111] 3.1 Activation of algal species and preparation of wastewater medium

[0112] The preparation of the activated and non-sterilized ultra-high ammonia nitrogen wastewater medium of thiaphophilic primordial red algae (Galdieria sulphuraria UTEX 2919) was carried out according to the methods described in 1.1 and 1.2, respectively.

[0113] 3.2 Four-stage fermenter culture

[0114] 3.2.1 Photofermentation culture: inoculate the red algae after 1.3 adaptive culture in Example 1 into non-sterile ultra-high ammonia nitrogen wastewater medium, and place it in a 5L fermenter (containing 3.5L wastewater medium) for cultivation . The initial inoculum density of photofermentation in red algae was 1×10 8 cells / ml, the pH value of the medium is 2.5, the temperature is 35°C, the stirring rate is 150r / min, the ventilation volume is 150L / h,...

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Abstract

The invention discloses a method for rapidly removing ammonia nitrogen by using a microalgae non-sterile fermentation method in an extreme environment and an application thereof. The method includes the following steps: S1, cultivating microalgae in extreme environments to the logarithmic growth phase to obtain seed liquid; S2, adding organic carbon sources to unsterilized ultra-high ammonia nitrogen industrial wastewater, and adjusting the pH value to 1- 4. Obtain an ultra-high ammonia nitrogen wastewater medium; wherein, the ammonia nitrogen concentration of the medium is higher than 2800mg / L; S3, inoculate the seed liquid after adaptive cultivation into a photofermentation tank equipped with an ultrahigh ammonia nitrogen wastewater medium for intermittent Feed-fed fermentation, when the ammonia nitrogen concentration in the light fermenter is lower than 20mg / L, update the wastewater medium to make the ammonia nitrogen concentration in the fermenter higher than 4700mg / L. The method of the invention has the advantages of high ammonia nitrogen removal efficiency, short treatment period, environmental protection, high-value phycobiliproteins can be recovered at the same time, and comprehensive development and utilization of resources are realized.

Description

technical field [0001] The invention belongs to the technical field of waste water treatment and resource utilization of waste materials, and in particular relates to a method for rapidly removing ammonia nitrogen by non-sterile fermentation of microalgae in an extreme environment and an application thereof. Background technique [0002] The rapid development of industry and agriculture has not only stimulated economic growth, but also brought a series of environmental pollution problems. A large amount of high ammonia nitrogen wastewater is produced in industrial and agricultural production, such as catalyst production wastewater, rare earth mining wastewater, pig farm biogas slurry and landfill leachate, etc. The concentration of ammonia nitrogen in such wastewater ranges from 400 to 10,000 mg / L or even higher. my country's current relevant environmental protection standards that involve ammonia nitrogen wastewater discharge indicators include "Surface Water Environmental ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/32C02F101/16
CPCC02F3/322C02F2101/16
Inventor 魏东朱宝君郑雅莉
Owner SOUTH CHINA UNIV OF TECH
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