PCR micro-reaction chamber array structure and method for packaging mixed liquid

Abstract

The invention discloses a PCR micro-reaction chamber array structure and a method for packaging mixed liquid. The array structure comprises a bottom plate, a cover plate and a middle layer arranged between the bottom plate and the cover plate, wherein micro-reaction chambers are formed in the middle layer, an oil seal cavity, a first flow channel and a second flow channel are formed in the cover plate; the first flow channel and the second flow channel communicate with the oil seal cavity; and each micro-reaction chamber communicates with the oil seal cavity through a throat, and the size of the throat enables the mixed liquid of the micro-reaction chambers to be isolated from one another through sealing oil in the oil seal cavity. According to the array structure, the mixed liquid can besimultaneously added into the micro-reaction chambers and isolated from one another, and then subsequent nucleic acid amplification reaction is facilitated.

Description

technical field [0001] The invention relates to a PCR microreaction chamber array structure and a method for encapsulating a mixed solution, belonging to the technical field of microfluidic chips. Background technique [0002] Biochip technology has developed rapidly in the past 20 years, especially the rapid development of microfluidic technology / new material technology and artificial intelligence technology, making biochip technology gradually industrialized. [0003] Most of the current PCR chips are large in size and require complex mechanical actions, and a chip can perform fewer detection items at the same time. For example, the gene expert kit (chip) of Saibei Company is relatively large, and one kit can only detect 4 to 6 items at the same time (such as US patent US9322052, patent US9669409). [0004] In the current PCR micro-reaction chamber array chip, it is often necessary to add the mixed solution while vacuuming during the inspection process (such as Chinese pa...

AI Technical Summary

Problems solved by technology

The entrance of the micro-reaction chamber of the micro-reaction chamber array of the FILMARRAY chip is pierced by a thin film, the thickness is shallow, and the substances in the micro-reaction chamber are more likely to overflow, thus causing pollution between the micro-reaction chambers

[0008] Other PCR micro-reaction chamber array chips often need to add the mixed solution while vacuuming, which requires an additional vacuum pump, which increases the workload of the operator

There are also PCR micro-reaction chamber array chips that require a special gas-permeable material to make, and need to be temporarily vacuumed, and the vacuuming process lasts for tens of minutes to an hour.

This requires configuration of additional vacuum pumps, increased operator workload and cost of chip fabrication

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