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Preparation method and application of high-concentration spore liquid of Ceriporia lacerata

A high-concentration technology of tearing cereus bacteria, applied in the field of agricultural microorganisms, can solve the problems of easily damaged cell structure, autolysis of mycelia, and affecting the production and production of cystic spores, so as to facilitate storage and reduce storage and transportation costs , Conducive to the effect of field spraying

Active Publication Date: 2020-11-13
四川金珠生态农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the prior art, the mycelium is extremely easy to destroy its complete cell structure after excessive accumulation, resulting in autolysis of the mycelium, which fundamentally affects the production and output of the cystic spores.

Method used

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  • Preparation method and application of high-concentration spore liquid of Ceriporia lacerata
  • Preparation method and application of high-concentration spore liquid of Ceriporia lacerata
  • Preparation method and application of high-concentration spore liquid of Ceriporia lacerata

Examples

Experimental program
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Effect test

preparation example Construction

[0057] The invention provides a method for preparing a high-concentration spore liquid of cereus cereus tearing, comprising the following steps:

[0058] Step 1, strain selection, select the strain of Ceriporia lacerata, which was preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on April 28, 2015, address: Beichen West Road 1, Chaoyang District, Beijing No. 3, Institute of Microbiology, Chinese Academy of Sciences, the preservation number is CGMCCNo.10485;

[0059] Step 2, slant seed culture:

[0060] After washing and slicing 200g of potatoes, boil them with an appropriate amount of deionized water for 30 minutes, filter with double-layer gauze, and take the filtrate for later use;

[0061] Weigh 20g of glucose and 20g of agar powder, dissolve in the above filtrate at the same time, heat and stir, after the agar powder is fully expanded and dissolved, use deionized water to make up to 1000ml, natural pH, and pack in Φ 2...

example 1

[0127] The air flow rate of the 500L fermentation tank VVM1=1:1.2vvm, D1=900mm, D2=2200mm is calculated by substituting empirical formula ①,

[0128] VVM2=VVM1×(D1 / D2) 2 / 3

[0129] =1.2×(900 / 2200) 2 / 3

[0130] =0.66

[0131] ≈0.7vvm.

[0132] Therefore, the air flow rate in the fermentation tank of the present invention is about 1:0.7, which is more suitable.

[0133] 2. Calculation of the speed of the fermentation tank

[0134] Empirical formula ②: N 2 =N 1 *(VVM 1 *V 1 *D 2 2 / VVM 2 *V2*D 1 2 ) 0.23 *(d 1 / d 2 ) 0.54

[0135] N1----speed of 500L fermenter VVM1----air flow of 500L fermenter

[0136] V1----filling volume of 500L fermenter D1----diameter of 500L fermenter

[0137] d1----the paddle diameter of the 500L fermentation tank

[0138] N2----rotational speed of 20t fermenter VVM1----air flow of 20tL fermenter

[0139] V1----the charging volume of the 20t fermenter D1----the diameter of the 20t fermenter

[0140] d1----the paddle diameter of the 20...

example 2

[0142] Air flow VVM1=1:1.2vvm of 500L fermentation tank, tank diameter D1=900mm, loading capacity V1=350L, speed N1=150rpm, paddle diameter d1=250mm;

[0143] 20t fermentation tank VVM1=1:0.7vvm, tank diameter D1=2200mm, loading V1=12000L, paddle diameter d2=670mm;

[0144] Substituting into empirical formula ② to calculate,

[0145] N 2 =150rpm×(1.22vvm×350L×2200 2 / 0.7vvm×12000L×900 2 ) 0.23 ×(250 / 670) 0.54 =150rpm×0.299 0.23 ×0.37 0.54

[0146] =67rpm

[0147] ≈70rpm

[0148] In actual use, it is necessary to judge whether the fermentation conditions are suitable according to the conditions of the fermentation tank site such as foam conditions, liquid level and tumbling conditions, etc.; based on 70rpm, the speed can be increased to 140 according to the situation on site. Or reduce to 60, so that the fermentor speed range is 60-140rpm.

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Abstract

The invention relates to the technical field of agricultural microorganisms, and discloses a preparation method and application of high-concentration spore liquid of Ceriporia lacerata. The preparation method comprises the following steps: culturing hyphae by seeds, and culturing in a fermentation tank to form fermentation tank mycelia; upon completion of fermentation, pretreating fermentation broth, adding a filter aid, uniformly stirring to obtain pretreated fermentation liquor, then feeding the pretreated fermentation liquor into a plate-and-frame filter press for filtering, discarding filtrate, drying and dehydrating an obtained filter cake to obtain solid mycelia, and then adding a spore protective agent, uniformly stirring and mixing to obtain a mycelium diluent, and finally subpackaging and hermetically storing the mycelium diluent to obtain the high-concentration spore liquid of the Ceriporia lacerata, which is convenient to store, capable of reducing the storage and transportation cost and more beneficial to field spraying and use. When mycelia are obtained from fermentation liquor, a filter aid is added, solid mycelia are obtained through drying and dehydration, and mycelium autolysis caused by excessive accumulation of the mycelia can be avoided.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a preparation method and application of a high-concentration spore liquid of A. cerevisiae. Background technique [0002] The use of chemical fertilizers and pesticides in my country ranks first in the world. The high input of chemical fertilizers and pesticides has brought about a series of environmental, ecological and food safety problems. In modern intensive agricultural production, a large number of frequent application of pesticides is required to prevent and control crop diseases. However, the long-term and large-scale application of pesticides has caused a series of production, environmental and health problems, such as enhanced drug resistance of bacteria, reduced drug efficacy, increased dosage, increased pesticide residues in the environment and agricultural products, affecting food safety, endangering human health, etc. . [0003] Fun...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00A01N63/30A01P3/00A01P21/00C12R1/645
CPCC12N1/14C12N3/00A01N63/30
Inventor 不公告发明人
Owner 四川金珠生态农业科技有限公司
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