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Cobalt-based mimic enzyme as well as preparation method and application thereof

A technology that simulates enzymes and cobalt bases. It is applied in chemical instruments and methods, nanotechnology for materials and surface science, and analysis by making materials undergo chemical reactions. It can solve problems such as poor biocompatibility and achieve catalytic decomposition. , The effect of excellent simulated enzyme characteristics and strong catalytic ability

Active Publication Date: 2020-11-13
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the biocompatibility of the simulated enzyme materials of these cobalt elements as disclosed above is relatively poor

Method used

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  • Cobalt-based mimic enzyme as well as preparation method and application thereof
  • Cobalt-based mimic enzyme as well as preparation method and application thereof
  • Cobalt-based mimic enzyme as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Preparation of cobalt-based nanozymes

[0048] 1) Add bovine serum albumin (hereinafter referred to as BSA) to a final concentration of 10 grams per liter into 0.1 moles per liter and a pH of 8 sodium bicarbonate buffer solution, stir and dissolve completely and take 6 milliliters of BSA in carbonic acid Sodium hydrogen solution, move in the 20 milliliter reaction bottle, add 0.6 milliliter concentration afterward and be the cobalt chloride solution of 0.4 mole per liter and mix and react for 5 minutes,

[0049] 2) Add 0.3 milliliters of glutathione aqueous solution with a concentration of 0.1 moles per liter to the solution obtained in the above step 1) to assist in the formation of crystal grains, then continue vigorously stirring at a constant temperature of 37 degrees Celsius for 4 hours, and then let it stand for 12 Hour aging followed by dialysis for 3 days using dialysis bags with a molecular weight cut-off of 10,000.

[0050] The prepared cobalt-based nanozyme ...

Embodiment 2

[0053] 1) Add ovalbumin to a sodium bicarbonate buffer solution with a final concentration of 20 grams per liter and a pH of 7.5 at a concentration of 0.1 mole per liter. After stirring and dissolving completely, take 6 milliliters of the sodium bicarbonate solution of BSA and transfer it to 20 milliliters In the reaction flask, add 0.3 ml of cobalt chloride solution with a concentration of 0.2 moles per liter and mix for 5 minutes;

[0054] 2) To the solution obtained in the above step 1), add 0.6 milliliters of cysteine ​​aqueous solution with a concentration of 0.05 moles per liter to assist the formation of crystal grains, then continue to stir vigorously at a constant temperature at 37 degrees Celsius for 6 hours, and then let it stand for 8 hours. Hour aging followed by dialysis for 2 days using a dialysis bag with a molecular weight cut off of 8000.

[0055] The cobalt-based nanozyme prepared in this example was also characterized by dynamic light scattering, and the av...

Embodiment 3

[0057] 1) Human serum albumin is added to the sodium bicarbonate buffer solution of 0.1 mole per liter and pH of 6 with a final concentration of 5 grams per liter, after stirring and dissolving completely, get the sodium bicarbonate solution of 6 milliliters of BSA, and move it into 20 In the milliliter reaction flask, add 0.3 milliliter concentration and be the cobalt sulfate solution of 0.05 mole per liter and mix and react for 10 minutes;

[0058] 2) To the solution obtained in the above step 1), add 0.6 milliliters of cysteine ​​aqueous solution with a concentration of 0.05 moles per liter to assist the formation of crystal grains, then continue to stir vigorously at a constant temperature at 37 degrees Celsius for 1 hour, and then let it stand for 12 Hour aging followed by dialysis for 1 day using a dialysis bag with a molecular weight cut-off of 15,000.

[0059] The cobalt-based nanozyme prepared in this example was also characterized by dynamic light scattering, and the...

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Abstract

The invention provides a cobalt-based mimic enzyme as well as a preparation method and application thereof, the cobalt-based mimic enzyme comprises cobalt-based nanoparticles and biological modification molecules compounded outside the cobalt-based nanoparticles, and the biological modification molecules comprise any one or a combination of at least two of biological protein molecules, glutathioneor cysteine molecules. The cobalt-based mimic enzyme has excellent mimic enzyme characteristics and can effectively catalyze decomposition of hydrogen peroxide.

Description

technical field [0001] The invention belongs to the technical field of biological materials, and relates to a cobalt-based mimetic enzyme, a preparation method and application thereof. Background technique [0002] Mimetic enzymes are a class of substances synthesized by chemical methods that have similar biological enzyme activity. They are mainly divided into organic polymer mimetic enzymes and nanomimetic enzymes (hereinafter referred to as nanozymes) developed with the development of nanoscience and technology in recent years. The essence of imitating enzymes is defined as a type of catalyst that is artificially synthesized with enzymatic properties. Compared with biological enzymes, it has simple structure, stable chemical properties, enzymatic functions, high efficiency, high selectivity, and low cost. Etc. The main structure of the nanomaterial enzyme that has been studied more is iron oxide nanoparticles. Based on the valence property of iron and other elements, it ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J31/28B01J35/02B82Y30/00B82Y40/00G01N21/78B01J35/00
CPCB01J31/003B01J31/28B82Y30/00B82Y40/00G01N21/78B01J35/40
Inventor 陈春英鲁婧怡王亚玲
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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