An elution machine for protein purification

Abstract

The present invention relates to an elution machine for protein purification. The technical scheme adopted is: including the body, the collection barrel rack, the purification column rack, the control system, the first peristaltic pump, the protein pretreatment device, the second peristaltic pump, the lateral movement device, the discharge valve, the mixing motor, the eluent adding Injection device, purification column, collection barrel, protein liquid injection pipeline, eluent injection pipeline. The invention designs a device capable of crushing the protein raw material and filtering to obtain the protein stock solution. The filtration adopts the methods of centrifugal and tangential flow filtration, which avoids the clogging of the filter screen during the filtration process, and has high filtration efficiency. At the same time, multiple groups of purification processing channels are added, which can automatically complete the configuration and filling of eluents with different concentrations according to the settings. The automatic multi-channel setting makes the purification and elution of proteins more convenient and can save a lot of money. It is convenient to carry out multiple sets of comparative experiments, and to study the elution effect of eluent and protein.

Description

technical field [0001] The invention belongs to the technical field of protein purification, and relates to an elution machine for protein purification. Background technique [0002] At present, protein purification systems are generally only suitable for process research operations in the laboratory. The degree of automation is low, and the operations of sample loading and washing cannot be carried out continuously. In the purification process, human factors have great influence and poor repeatability. At the same time, the traditional operation is to fix the protein purification column on the iron frame, hold the sample addition tube containing the sample addition container in one hand, and hold the pipette in the other hand for sample addition, which has the problems of inconvenient operation, time-consuming and laborious operation, etc. Protein purification control equipment has been developed in the prior art, but most of them are single-channel independent control, whi...

AI Technical Summary

Problems solved by technology

[0002] At present, protein purification systems are generally only suitable for laboratory process research operations. The degree of automation is low, and it is often impossible to continuously perform sample loading and washing operations. In the purification process, human factors have a great influence and poor repeatability.

At the same time, the traditional operation is to fix the protein purification column on the iron stand, hold the sample tube containing the sample container in one hand, and add the sample with the pipette in the other hand, which has problems such as inconvenient operation, time-consuming and labor-intensive, although In the prior art, protein purification control equipment has been developed, but most of them are single-channel single-control, which cannot be used for large-scale and large-capacity protein purification. Eluent or different types of eluents are used to analyze the elution effect, which requires easy adjustment of the eluent and the addition of multiple sets of elution columns for elution, so as to facilitate research

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