Eluting machine for purifying proteins

Abstract

The invention relates to an eluting machine for purifying proteins. The adopted technical scheme is as follows: the eluting machine comprises a machine body, a collecting bucket frame, a purifying column frame, a control system, a first peristaltic pump, a protein preprocessing device, a second peristaltic pump, a transverse moving device, a discharging valve, a hybrid motor, an eluant filling device, a purifying column, a collecting frame, a protein fluid filling pipeline and an eluant filling pipeline. A device capable of acquiring protein raw fluid through crushing protein raw materials andcarrying out filtration is designed and adopts a centrifugal filtration manner and a tangential flow filtration manner, so that the situation that a filtering net is blocked in the filtering processis avoided, and the filtering efficiency is relatively high. Meanwhile, by additionally forming multiple purifying processing channels, preparation and filling of eluants with different concentrationscan be automatically finished according to settings; and by virtue of automatic configuration of the multiple channels, purification and elution of the proteins are more convenient and faster, a lotof labor time can be saved, human errors can be effectively avoided, multiple groups of contrast experiments are convenient to carry out, and elution of the eluent and the proteins are convenient to research.

Description

technical field [0001] The invention belongs to the technical field of protein purification and relates to an eluting machine for protein purification. Background technique [0002] At present, protein purification systems are generally only suitable for laboratory process research operations. The degree of automation is low, and it is often impossible to continuously perform sample loading and washing operations. In the purification process, human factors have a large impact and poor repeatability. At the same time, the traditional operation is to fix the protein purification column on the iron stand, hold the sample tube containing the sample container in one hand, and add the sample with the pipette in the other hand, which has problems such as inconvenient operation, time-consuming and labor-intensive, although In the prior art, protein purification control equipment has been developed, but most of them are single-channel single-control, which cannot be used for large-sc...

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Problems solved by technology

[0002] At present, protein purification systems are generally only suitable for laboratory process research operations. The degree of automation is low, and it is often impossible to continuously perform sample loading and washing operations. In the purification process, human factors have a great influence and poor repeatability.

At the same time, the traditional operation is to fix the protein purification column on the iron stand, hold the sample tube containing the sample container in one hand, and add the sample with the pipette in the other hand, which has problems such as inconvenient operation, time-consuming and labor-intensive, although In the prior art, protein purification control equipment has been developed, but most of them are single-channel single-control, which cannot be used for large-scale and large-capacity protein purification. Eluent or different types of eluents are used to analyze the elution effect, which requires easy adjustment of the eluent and the addition of multiple sets of elution columns for elution, so as to facilitate research

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