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Flocculating yeast bioadhesive as well as preparation method and use method thereof

A bioadhesive and flocculation technology, applied in biochemical equipment and methods, microbial-based methods, adhesive types, etc., can solve the problem of expensive mussel adhesion protein, high cost of adhesion protein extraction, and limited biological adhesion In order to achieve low irritation, low antibacterial effect and reduce the release of formaldehyde

Pending Publication Date: 2020-10-30
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation of mussel adhesive proteins by biological extraction is expensive, which limits the application of bioadhesives.
On the one hand, the cost of extracting mussel adhesion protein is high, such as a method of extracting adhesion protein from emerald mussel, which has good biocompatibility and waterproof bioadhesive, as the method of directly extracting adhesion protein from organisms, the cost is somewhat Reduced, but the adhesive protein prepared by it is still relatively expensive as a raw material for adhesives

Method used

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  • Flocculating yeast bioadhesive as well as preparation method and use method thereof
  • Flocculating yeast bioadhesive as well as preparation method and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1D

[0053] Example 1 The extraction method of the protein rich in Dopa content:

[0054] Take the feet of live marine mussels, wash them with PBS at -5°C to 0°C, chop them in a 5% acetic acid solution containing protease inhibitors at -5°C to 0°C, and chop the above materials with a disperser , the mixture was homogenized on ice;

[0055] The treated tissue homogenate was centrifuged, and the supernatant was separated, and 60% perchloric acid (PCA) was slowly added to the supernatant in an ice bath at the same time to a final concentration of 1%, stirred for 20-40 minutes, centrifuged, and recovered. Serum;

[0056] Cool the supernatant in -10°C to -20°C ice ethanol, add concentrated sulfuric acid to acidify to a final concentration of 0.3mmol / L while stirring, add twice the volume of -82°C to Acetone at -86°C;

[0057] After standing still for 20-30 minutes, centrifuge, discard the supernatant, take the precipitate and dissolve it in 5% acetic acid, centrifuge again, take the ...

Embodiment 2

[0058] Example 2 Preparation of flocculated yeast bioadhesive

[0059] Preparation of flocculated yeast bioadhesives as figure 1 As shown in the flow chart of the preparation of the flocculated yeast bioadhesive, the flocculated Saccharomyces cerevisiae is factory-produced through a fermenter 1, and the tank body of the fermenter 1 is provided with a baffle, an aeration and a stirring blade 2. The cultured flocculated Saccharomyces cerevisiae 3 can be prepared into two forms of bioadhesives, namely liquid and solid. When the cells are settled, a liquid bioadhesive can be obtained; when the cells are made into a dry powder, they are mixed with Dopa-rich protein and polysaccharide mixed dry powder to make a solid bioadhesive.

[0060] 1. The preparation process of liquid flocculation yeast bioadhesive:

[0061] Take the flocculated Saccharomyces cerevisiae cryopreservation solution from the refrigerator at -80°C, inoculate it into YPD seed medium (1% yeast extract, 2% peptone,...

Embodiment 3

[0069] Embodiment 3 liquid bioadhesive or activated solid bioadhesive is used to prepare plywood

[0070] Rotate the wood section material into 1-2mm veneers with a rotary cutter, and dry the veneers with a hot air stove until the water content is 10-12%, and mix carbon fiber powder and liquid wear-resistant rubber by 0.05-0.1: The weight ratio of 1 is evenly mixed to obtain a wear-resistant coating;

[0071] The above-mentioned wear-resistant coating is evenly coated on the prepared veneer, and air-dried naturally, the amount of the wear-resistant coating is 100-200g / m 2 ;

[0072] Use a glue applicator to coat the adhesive on both sides of the prepared veneer, and the amount of flocculated yeast bioadhesive is 280-300g / m 2 , the flocculated yeast bioadhesive is a liquid bioadhesive or an activated solid bioadhesive, after gluing, it is assembled, and the veneers after gluing are arranged in a criss-cross pattern and bonded together according to the wood texture structure, ...

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Abstract

The invention discloses a flocculating yeast biological adhesive as well as a preparation method and a use method thereof, and relates to the field of biological adhesives. The flocculating yeast biological adhesive comprises protein, polysaccharide and flocculation yeast living cells, wherein the protein contains mussel protein dopa (Dopa), and the flocculating saccharomyces cerevisiae comprisesone or more of S.cerevisiae SPSC01, S.cerevisiae 6525 Flol and S.cerevisiae 4126 Flol; the preparation method comprises the steps of activation, culture, collection and mixing. The liquid state is suitable for in-situ use; the solid state is suitable for long-term storage and transportation. The Dopa endows the adhesive with strong adhesion efficiency, the flocculating yeast is used for adjustingthe adhesion strength, and meanwhile, the active cells enable the adhesive to continuously perform biochemical reaction for a period of time after adhesion. The biological adhesive is good in biological adhesive property, has waterproof, low-irritation and antibacterial properties, and can reduce formaldehyde release.

Description

technical field [0001] The invention relates to the field of bioadhesives, in particular to a flocculated yeast bioadhesive and methods for its preparation and use. Background technique [0002] The condensation of formaldehyde and urea can form urea-formaldehyde resins, so-called resin glues. Due to the low price of raw materials, this type of glue is widely used, mainly in the production of wooden board materials. Urea-formaldehyde resin contains unreacted formaldehyde, which is located in various parts of wood-based panels, and the release of formaldehyde in the deep layer is a slow and continuous process, which has caused the problem of formaldehyde pollution in indoor building materials and office appliances. People living indoors are prone to long-term exposure to formaldehyde, which is harmful to human health. Therefore, from the perspective of environmental protection, bio-adhesives that do not release formaldehyde appear to partially replace urea-formaldehyde resi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09J105/00C09J189/00C09J11/06C12N1/18C12R1/865
CPCC09J11/06C09J105/00C12N1/18C08L89/00C08K5/1545
Inventor 刘晨光冯颀张家威
Owner SHANGHAI JIAO TONG UNIV
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