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A kind of yeast living cell facial mask and its preparation method and application method

A technology of Saccharomyces cerevisiae cells and live cells, which is applied in the field of yeast live cell mask and its preparation. It can solve the problems of high price of SKII series and difficulty in daily use of ordinary households, and achieve the removal of chemical residues, stable flocculation characteristics, and cost reduction. Effect

Active Publication Date: 2021-03-30
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high price of the SKII series, it is difficult for ordinary families to use it daily

Method used

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  • A kind of yeast living cell facial mask and its preparation method and application method
  • A kind of yeast living cell facial mask and its preparation method and application method
  • A kind of yeast living cell facial mask and its preparation method and application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The preparation of the facial mask of embodiment 1, facial mask paper form

[0051] Pick the flocculated Saccharomyces cerevisiae frozen storage solution from the -80°C refrigerator, inoculate it into the YPD seed culture solution (1% yeast extract, 2% peptone, 2% glucose, and sterilize at 121°C for 20 minutes) for activation;

[0052] When the yeast cell growth reaches the middle and late stages, 1% of the activated seed culture liquid is transferred to the improved YPD fermentation culture liquid (in the YPD seed culture liquid, aromatic amino acids, vitamins, mannose are additionally added, and sterilized, wherein the vitamin Sterilize by filtration) culture;

[0053] Collect the flocculated Saccharomyces cerevisiae cells in the lower layer of the fermenter when the yeast cells reach the middle stage of growth (the OD600 value measured after the flocculation with EDTA hydrolyzed solution is about 2.0);

[0054] Yeast cells are collected and made into a mask with a c...

Embodiment 2

[0055] Embodiment 2, the preparation of the facial mask of mud mask form

[0056] It is basically the same as the above example, except that the collected yeast cells are made into a clay mask instead of a mask paper.

Embodiment 3

[0057] Embodiment 3, the preparation and application method of the facial mask of dry cell powder form

[0058] Pick the flocculated Saccharomyces cerevisiae frozen storage solution from the -80°C refrigerator, inoculate it into the YPD seed culture solution (1% yeast extract, 2% peptone, 2% glucose, and sterilize at 121°C for 20 minutes) for activation;

[0059] When the yeast cell growth reaches the middle and late stages, 1% of the activated seed culture medium is transferred to the YPD fermentation medium (1% yeast extract, 2% peptone, 2% glucose, sterilized at 121° C. for 20 minutes) for cultivation;

[0060] When the yeast cells reach the middle stage of growth (the OD600 value measured after changing to EDTA hydrolyzate is about 2.0), the flocculated Saccharomyces cerevisiae cells in the lower layer of the fermenter are collected, and the yeast is made into a dry powder by using a freeze dryer;

[0061] Stir the flocculated Saccharomyces cerevisiae cell dry powder and t...

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Abstract

The invention discloses a yeast living cell facial mask, which comprises flocculated living cells of Saccharomyces cerevisiae. In particular, the flocculated S. cerevisiae comprises one or more of Saccharomyces cerevisiae SPSC01, Saccharomyces cerevisiae 6525Flo1 and Saccharomyces cerevisiae 4126Flo1. The invention also discloses the preparation method and application method of the facial mask. The surface of flocculated Saccharomyces cerevisiae cells is fluffy and porous, with a large specific surface area; it is rich in reducing groups such as hydroxyl and aldehyde groups, which can restore high-valent heavy metal ions on the skin surface; it is rich in protein, vitamins, amino acids and organic acids; and the main functional components of SKII Compared with pitera, living cells can continuously and efficiently produce reducing power, which helps to slow down the aging of skin cells.

Description

technical field [0001] The invention relates to the field of skin care products, in particular to a yeast living cell facial mask and a preparation method and application method thereof. Background technique [0002] In recent years, people's demand for skin care has become increasingly strong, and the sales of skin care products have grown steadily year by year. Among the many skin care products, the SKII series produced by Procter & Gamble in Japan is very popular. The main component of SKII is Pitera, which is a metabolic fluid extracted from the culture and fermentation of Saccharomycopsis yeast in a low-oxygen environment. Its main components include vitamins, amino acids, minerals and organic acids. Wait. However, due to the high price of the SKII series, it is difficult for ordinary families to use them daily. Therefore, be badly in need of a kind of low-cost, good facial mask of effect, bring gospel to the vast crowd that needs skin care. Contents of the inventi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9728A61Q19/00A61Q19/10C12N1/18C12R1/865
CPCA61K8/99A61Q19/00A61Q19/10C12N1/18
Inventor 刘晨光曹莲莹闻远覃锦程李凯夏娟
Owner SHANGHAI JIAO TONG UNIV
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