Medium- and long-chain fatty acid grease rich in 1, 3-dilaurate-palmitic acid triglyceride and preparation and application thereof

A technology of palmitic acid triglyceride and medium and long-chain fatty acids, which is applied in the field of bio-manufacturing of functional oils and fats, can solve the problems of no characteristics of raw edible oils, no site specificity of solid acid catalysts, no richness, etc., and achieve good results. Application value, promotion of body weight gain, effect of low requirements on reaction conditions

Pending Publication Date: 2020-10-30
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solid acid catalyst is not site-specific, and the method does not pursue the insertion of a specific medium-chain fatty acid into a specific site, and the raw material edible oil has no distinctive characteristics, and the synthesized target product does not have rich 1,3-dilauric acid- 2-palmitic acid triglyceride this characteristic

Method used

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  • Medium- and long-chain fatty acid grease rich in 1, 3-dilaurate-palmitic acid triglyceride and preparation and application thereof
  • Medium- and long-chain fatty acid grease rich in 1, 3-dilaurate-palmitic acid triglyceride and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Add 5.0g of palm stearin and 12.12g of lauric acid (molar ratio is 1:10) into a 100ml round bottom flask, fill it with nitrogen, cover with an inversion plug, and place it in a constant temperature water bath shaker at 65°C for a period of time, After the reaction substrate is mixed evenly and in a liquid state, add 1.712g (w / w=10%) of AO IM lipase (provided by Hangzhou Kangyuan Food Technology Co., Ltd.), then fill with nitrogen protection, cover the opening plug, React for 2.5h under the condition of 200r / min.

[0025] (2) After the reaction, remove the immobilized enzyme by filtration, collect the mixed material, use KOH to neutralize the free fatty acid, extract the glyceride with n-hexane, and then distill and remove the n-hexane to obtain the target product. The obtained product was determined by a gas chromatography spectrometer, and the content of 1,3-dilauric acid-2-palmitic acid triglyceride was 39.59%.

[0026] The mass spectrum of 1,3-dilauric acid-2-pa...

Embodiment 2

[0032] (1) Add 5.0g of palm stearin and 9.7g of lauric acid (molar ratio: 1:8) into a 100ml round bottom flask, fill it with nitrogen, cover with an inversion plug, and place it in a constant temperature water bath shaker at 60°C for a period of time. After the reaction substrates were mixed uniformly and in liquid state, 1.178g RM IM lipase (w / w=8%) was added, then flushed with nitrogen protection, covered with an inversion plug, and reacted at 200r / min for 7h.

[0033] (2) After the reaction, remove the immobilized enzyme by filtration, collect the mixed material, use KOH to neutralize the free fatty acid, extract the glyceride with n-hexane, and then distill and remove the n-hexane to obtain the target product. The obtained product was determined by a gas chromatography spectrometer, and the content of 1,3-dilauric acid-2-palmitic acid triglyceride was 31.76%.

Embodiment 3

[0034] Example 3 (1) Add 5.0 g of palm stearin and 9.7 g of lauric acid (the molar ratio is 1:8) in a 100 ml round bottom flask, fill with nitrogen, cover with an inversion plug, place in a constant temperature water bath shaker at 60°C After a period of time, after the reaction substrate is mixed uniformly and becomes liquid, add 1.178g TL IM lipase (w / w=8%), then flush with nitrogen protection, cover with an inversion plug, and react at 200r / min for 16h.

[0035] (2) After the reaction, remove the immobilized enzyme by filtration, collect the mixed material, use KOH to neutralize the free fatty acid, extract the glyceride with n-hexane, and then distill and remove the n-hexane to obtain the target product. The obtained product was determined by a gas chromatography spectrometer, and the content of 1,3-dilauric acid-2-palmitic acid triglyceride was 24%.

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Abstract

The invention discloses a medium- and long-chain fatty acid grease rich in 1, 3-dilaurate-palmitic acid triglyceride and preparation and application of the medium- and long-chain fatty acid grease. The preparation method comprises the following steps of: lauric acid and palm stearin are used as substrates, sn-1, 3-site specific lipase is used as a catalyst to perform acidolysis reaction, after thereaction is finished, the catalyst and free fatty acid are removed to obtain the medium and long-chain fatty acid grease, wherein the content of 1, 3-dilaurate-palmitic acid triglyceride in the medium- and long-chain fatty acid grease is 20-40%. The invention further discloses application of the medium- and long-chain fatty acid grease rich in 1, 3-dilaurate-palmitic acid triglyceride in weight gain of special crowds or animals.

Description

technical field [0001] The invention relates to the field of biological production of functional oils, in particular to a preparation method and application of a medium-long chain fatty acid oil rich in 1,3-dilauric acid-2-palmitic acid triglyceride. Background technique [0002] Oil is an important part of food. Its chemical structure is mainly the ester formed by glycerol and fatty acid. As one of the indispensable nutrients for the body, it must provide energy for the body on the one hand, and play a special role in health on the other hand. The type and location of fatty acids in triglycerides affect their metabolism. Medium-chain fatty acid (MCT) metabolizes faster than long-chain fatty acid (LCT). MCT can be hydrolyzed quickly and absorbed into the portal vein, while LCT is hydrolyzed slowly. It needs to be combined with chylomicrons, transported through the lymphatic system into the circulatory system for absorption. The MLM type (Middle-long-middle) in medium and lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/12A23K20/158C12P7/64
CPCA23L33/12A23K20/158C12P7/6445A23V2002/00A23V2200/30A23V2250/1944A23V2250/1942
Inventor 冯凤琴张辉李闯
Owner ZHEJIANG UNIV
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