Application of chickpea extract in prevention and treatment of pathogenic bacteria of crops
A technology of pathogenic bacteria and crops, applied in the agricultural field, can solve problems such as excessive pesticide residues, adverse effects on humans, animals and the ecological environment, and threats to human health
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Embodiment 1
[0022] Isolation and Identification of Anti-Xag Active Substance Extracts from Chickpea Plants
[0023] Weigh 1g of chickpea plant, cut it into small pieces with a length of about 1mm with a knife, transfer it to a mortar and grind it, transfer the tissue fragments to a centrifuge tube, add 200μL of ethanol to it, and shake it in a shaker at 12000rpm for 10min , transfer the centrifuge tube to a 4°C centrifuge at 8000rpm, centrifuge for 10min, filter the supernatant through a 0.22μm filter membrane and detect it with high performance liquid chromatography. Adopt C18 chromatographic column (250mm * 4.6mm, Phenomenex), the ultraviolet detection wavelength is 254nm, with acetonitrile and water as mobile phase, the content of acetonitrile is gradually increased from 10% to 100% in the first 30min, after maintaining 5min, drop to 10% immediately and Maintain for 5 minutes, the flow rate is 1 mL / min, and the temperature of the column oven is 25°C. High performance liquid chromatogr...
Embodiment 2
[0028] Detection of antagonistic activity of biochanin A and genistein against pathogenic bacteria in crops
[0029] The crop pathogenic bacteria used in this example include Xag, Ea, Cm, and Ac. The preparation method of the pathogenic bacteria liquid is the same as in Example 1. Add 4 μL bacterial solution to 200 μL LB liquid medium containing 0 μg / mL, 50 μg / mL, 100 μg / mL, 200 μg / mL, 300 μg / mL, 400 μg / mL, 500 μg / mL chickpein A or genistein, The turbidity of the bacterial solution was monitored after 6 hours of shaking culture at 200 rpm in a shaker at 28°C. The results are shown in Table 1.
[0030] Table 1 Minimum inhibitory concentration of biochanin A and genistein to pathogenic bacteria in crops
[0031]
[0032] It can be seen from the above table that the minimum inhibitory concentration (MIC) of biochanin A to Xag is 100 μg / mL, and the MIC value to Ea, Cm, and Ac is 200 μg / mL. The MIC of genistein to Xag, Ea, Cm, and Ac is greater than 500 μg / mL. It can be see...
Embodiment 3
[0034] Effects of biochanin A on the motility of Xag flagella
[0035] Xag bacterial liquid preparation method is the same as embodiment 1. Prepare LB solid medium containing 0 μg / mL, 5 μg / mL, 12.5 μg / mL, 25 μg / mL, and 50 μg / mL chickpein A, add 3 μL of bacterial solution to the medium, and place the culture dish at 28 After culturing for 72 hours in an incubator at ℃, measure the colony diameter.
[0036] The result is as figure 2 As shown, 50 μg / mL biochanin A can significantly inhibit the flagellar mobility (Swimmingmotility) of Xag.
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