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High performance liquid detection method of surfactant triton X-100

A technology of X-100 and surfactant, applied in the field of high-efficiency liquid phase detection of Triton X-100, can solve the problems of cumbersome operation, general accuracy, and low detection sensitivity

Pending Publication Date: 2020-10-27
ZHEJIANG PHARMA COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to provide a surfactant triton X-100 high-performance liquid phase detection with good separation effect, high precision and accuracy, safe and simple operation, convenient and fast processing, suitable for high-throughput screening and accurate quantification method to solve the problems of low detection sensitivity, cumbersome operation and general accuracy in the above conventional detection methods

Method used

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  • High performance liquid detection method of surfactant triton X-100
  • High performance liquid detection method of surfactant triton X-100
  • High performance liquid detection method of surfactant triton X-100

Examples

Experimental program
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Embodiment 1

[0046] The experimental conditions in the embodiments of the present invention are as follows:

[0047] Instrument: high performance liquid chromatography (Shimadzu LC-20AT);

[0048] Chromatographic column: Waters XBridge C18 chromatographic column, the column length is 250mm;

[0049] Need test solution: Accurately weigh 10.0 mg of Triton X-100 test substance, place it in a 10ml volumetric flask, dissolve and constant volume with mobile phase (methanol:water=90:10), to obtain the test solution;

[0050] Flow rate: 1.0ml / min;

[0051] ELSD detector atomization temperature: 45°C;

[0052] ELSD detector carrier gas pressure: 26psi;

[0053] Mobile phase: methanol / water=90 / 10 (v / v).

[0054] Based on the above-mentioned experimental conditions, the inventor also provides a high-efficiency liquid phase method for determining the content of Triton X-100, which is carried out in the following steps:

[0055] (1) Preparation of reference substance solution:

[0056] Accurately...

Embodiment 2

[0068] Instrument: high performance liquid chromatography (Shimadzu LC-20AT);

[0069] Chromatographic column: Waters XBridge C18 chromatographic column, the column length is 250mm;

[0070] Blank solution: mobile phase solution;

[0071] Flow rate: 1.0ml / min;

[0072] ELSD detector atomization temperature: 45°C;

[0073] ELSD detector carrier gas pressure: 26psi;

[0074] Mobile phase: methanol / water=90 / 10 (v / v).

[0075] Through 10 consecutive blank solution measurements, the peak area mean and standard deviation were obtained, and the value of the 10 blank peak area mean minus 3 times the standard deviation was substituted into the working curve of formula 1 to obtain a minimum detection limit of 0.5 μg / ml, compared with The detection sensitivity is doubled with a UV detector.

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Abstract

The invention discloses a high performance liquid detection method of triton X-100. A reversed-phase C18 chromatographic column and an ELSD detector are adopted, a mobile phase is a mixed solution ofmethanol and water, and isocratic elution is adopted. According to the method, 5-20 [mu]l of a sample is injected, triton X-100 can be effectively detected, the lowest detection concentration is 0.5 [mu]g / ml, the HPLC spectrum baseline is stable, and drifting does not occur. The method can be used for determining the content of triton X-100, has the advantages of high sensitivity, good linear relationship, good reproducibility, high precision and high accuracy, and has important research value in the aspects of industrial analysis, product formula research and the like.

Description

technical field [0001] The invention relates to the technical field of industrial analysis, in particular to a high-efficiency liquid phase detection method for Triton X-100. Background technique [0002] Triton X-100 (English: Triton X-100) is a non-ionic surfactant, which can dissolve the cell membrane in immunocytochemistry by dissolving the lipid components on the cell membrane, so that the antibody can enter the cell and bind to the antigen. With a hydrophilic polyethylene glycol chain (n is usually 9 or 10) and a lipophilic hydrocarbon group (4-(1,1,3,3-tetramethylbutyl)-phenyl group). Triton X-100 does not dissociate in water, has high stability in solution, is not easily affected by strong electrolyte inorganic salts, and can combine with lipids such as phospholipids in biological membranes to form soluble complexes; the hydrophobic end can also be combined with The hydrophobic regions of the membrane proteins combine to form complexes that dissolve in solution. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/36G01N30/86
CPCG01N30/02G01N30/06G01N30/36G01N30/8679
Inventor 宋文芳
Owner ZHEJIANG PHARMA COLLEGE
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