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Human staphylococcus as well as culture method and application thereof

A staphylococcus and culture method technology, applied in the field of microorganisms, can solve problems such as large side effects and unsatisfactory effects of pertussis drugs, achieve high safety, improve respiratory flora, and avoid intestinal diseases.

Active Publication Date: 2020-10-27
深圳微健康基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a human staphylococcus and its culture method and application, aiming to solve the technical problems of unsatisfactory effects and large side effects of existing pertussis medicines

Method used

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  • Human staphylococcus as well as culture method and application thereof
  • Human staphylococcus as well as culture method and application thereof
  • Human staphylococcus as well as culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Isolation, purification, screening and identification of Staphylococcus hominis WHG2019031202

[0040] 1) Isolation and purification of Staphylococcus hominis WHG2019031202

[0041] ① Select 10 healthy children. After opening their mouths and exposing their throats with an "ah" sound, use a disposable sterile swab to wipe the secretions from the palatal arches, pharynx and tonsils on both sides with sensitive and gentle movements;

[0042] ② Smear the swab carrying throat secretions on the blood agar plate medium (hereinafter referred to as the blood plate) in a Z-shaped line, put it upside down in a constant temperature incubator at 35°C, and incubate for 24 hours;

[0043] ③Observe and record the colony growth after 24 hours;

[0044] ④ Use a disposable inoculation loop to pick individual colonies of different forms on the blood plate, inoculate them one by one with the three-section line method, and put them upside down into a 35°C constant temperature incubator for...

Embodiment 2

[0072] Determination of the antibacterial activity of the fermentation broth of human Staphylococcus hominis WHG2019031202 strain

[0073] 1) Prepare the sterile filtrate of the fermentation broth

[0074] ① Pick a bacterial lawn of Staphylococcus hominis WHG2019031202 purified from a bacterial loop, inoculate it into a test tube containing 10 mL of broth medium, put it in a constant temperature incubator at 35°C, and cultivate it for 24 hours. The obtained bacterial suspension is used as the seed liquid .

[0075] Broth medium: peptone 10.0g, beef extract 3.0g, NaCl 15.0g, 1L distilled water, pH7.0-7.2.

[0076] ②Put the seed solution into five 100mL Erlenmeyer flasks containing 50mL basic fermentation medium according to the proportion (V / V) of 2‰, 4‰, 6‰, 8‰, and 10‰, and mark them accordingly. 35°C, 150r / min, shaking culture for 72h.

[0077] Basic fermentation medium: 20.0g glucose, 20.0g tryptone, 5.0g NaCl, KH 2 PO 4 1.0g, K 2 HPO 4 2.0g, 1L distilled water, pH7...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to human staphylococcus as well as a culture method and application thereof. The human staphylococcus is preserved in the Guangdong Microbiological Culture Collection Center, and the preservation number of the human staphylococcus is GDMCC No. 60608. The human staphylococcus has high safety for treatment of pertussis diseases, can improve respiratory tract flora and avoid other intestinal diseases, can be applied to infants or adults, has a wide application range, and provides a new selection mode for treatment of pertussis diseases in the future.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a human staphylococcus and a culture method and application thereof. Background technique [0002] Whooping cough (Pertussis), also known as "Mandarin duck cough" and "epidemic cough" among the people, is an acute respiratory infectious disease caused by the pertussis bacilli. The clinical feature is that the cough gradually worsens, showing a typical paroxysmal, spasmodic cough, and a deep and long cock-like inspiratory roar at the end of the cough. The course of the disease lasts for 2-3 months, so it is called pertussis. The source of pertussis infection can be children with bacteria. Young people who are infected have a high rate of missed diagnosis and have no obvious symptoms. They are important sources of infection. Pertussis bacillus is mainly transmitted by droplets. Symptoms appear after the incubation period (about 7-10 days), and people who lack sp...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/741A61P31/04A61P11/14C12R1/44
CPCC12N1/20A61P31/04A61P11/14A61K2035/115C12R2001/44C12N1/205
Inventor 戴文魁周潜李嘉琪黄琳娣薛俊免周乐天栗东芳杨振宇李寅虎
Owner 深圳微健康基因科技有限公司
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