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Determination method of microporous carrier detection inhibitor

A microporous carrier and assay method technology, applied in the field of phytopathology, can solve the problems of omission of inhibitory active substances, large sample volume, easy to miss, etc.

Active Publication Date: 2020-10-23
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This assay technical solution requires a relatively large sample volume of the sample to be tested. When the sample volume is too small, it is often impossible to detect, and it is easy to miss or omit inhibitory active substances with small content.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Apply a kind of measuring method of microporous carrier detection inhibitor of the present invention, measure the inhibitory action of medicine 25% benzazidine to the germination of the spore germination of Fusarium wilt bacterial strain Fo-4, carry out operation according to the following steps:

[0037] 1. Preparation of qualified microporous carriers with microplates

[0038] 1) Stabilization of the microplate: prepare a surface-clean clamping tool that can securely and flatly place the microplate, transfer it to a sterile workbench, and clamp the clean microplate onto the clamping tool.

[0039] 2) Melting of the agar medium: heat and melt the potato agar medium suitable for the germination of the spores of Fusarium wilt fungus contained in a triangular flask.

[0040] 3) Disposal of the culture medium at 80°C: pre-heat the ordinary water bath to a constant temperature of 80°C, put the melted medium in operation 2) into the water bath, and equilibrate to 80°C.

[0...

Embodiment 2

[0054] Apply a kind of assay method of microporous carrier detection inhibitor of the present invention, measure the inhibitory effect of drug 25% propiconazole to the spore germination of rice false smut bacterial strain Uv-111, carry out by step 1 to step 5 operation of embodiment 1, different What is more important is that the drug sample loaded in step 2 is a 500-fold dilution of 25% propiconazole, and the spores loaded in step 3 have a concentration of 10 6 spore / ml of the rice smut bacteria strain Uv-111 spore liquid, the cultivation time of step 4 is 48 hours.

[0055] Results The spore germination rate of sterile water blank control treatment was 95.40%, while the germination rate of propiconazole treatment was 0; the inhibitory effect of propiconazole samples on rice smut spores was 100%.

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Abstract

The invention discloses a determination method of a microporous carrier detection inhibitor. 40 microliters of agar culture medium is injected into micropores of a microporous plate to prepare a microporous carrier, a drug sample and pathogenic bacteria spores are loaded on the microporous carrier, and the inhibition effect of the drug on spore germination is detected. The determination method comprises the following steps: 1) preparing a qualified microporous carrier by using a microporous plate; 2) loading the sample to be measured into the microporous carrier; 3) loading the pathogenic bacteria spores into the microporous carrier; 4) carrying out spore germination culture; and 5) observing results. The method has the advantages that: 1) 96 microporous carriers can be prepared through one conventional microporous plate, miniaturization of a test system can be achieved; and 2) only 10 microliters of sample liquid is consumed by one test unit, and micronization of detected samples is achieved.

Description

technical field [0001] The invention relates to plant pathology technology, in particular to a method for measuring inhibitors detected by a microporous carrier. [0002] technical background [0003] At present and for a long time to come, pesticide control is still the main means of plant disease control. Exploring and excavating biological source pesticides is an important direction of pesticide control, and the early exploration and excavation of biological source pesticides often requires collecting or collecting substances that have inhibitory effects on plant pathogenic bacteria from nature, or isolating them from organisms or biological products that have inhibitory effects active ingredients. Obviously, efficient and feasible inhibitor identification and detection methods will help improve the effectiveness and efficiency of biogenic pesticide exploration and mining. The current common method for identifying the effect of inhibitors on pathogenic bacteria includes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/20
CPCC12Q1/20
Inventor 张君成王忠文张正淳
Owner GUANGXI UNIV
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