Hemolysin for blood analysis and preparation method thereof and reagent

A technology of blood analysis and hemolysin, which is applied in the field of blood analysis, can solve the problems of not being able to achieve the function of hemolysin multi-type flow cytometry, not being able to meet normal use in time, differences in mononuclear and granulocyte grouping, etc., and achieve a wide range of applications , low cost of use, and obvious effect of partitioning

Inactive Publication Date: 2020-10-16
四川恒健生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the hemolysin used for blood analysis in my country is mostly imported original reagents, which have disadvantages such as high cost and cumbersome purchase channels, which cannot meet the normal use of domestic hospitals in time, and the formula and instruments of hemolysin used for blood analysis are limited. There is a certain relationship. Different instruments use different hemolysins for blood analysis. The same hemolysin for blood analysis has different effects on different instruments. The main reason is the shape of white blood cells after hemolysin is lysed for blood analysis. There are differences, combined with the optical path design of FSC / SSC, it will lead to differences in lymphoid, mononuclear, and granulocyte grouping
At present, many hemolysins used for blood analysis on the market are only suitable for a certain type of flow cytometer, and cannot achieve the role of a hemolysin universal multi-type flow cytometer

Method used

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  • Hemolysin for blood analysis and preparation method thereof and reagent
  • Hemolysin for blood analysis and preparation method thereof and reagent
  • Hemolysin for blood analysis and preparation method thereof and reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Specifically, a reagent for blood analysis including hemolysin and staining solution for blood analysis.

[0034] In this embodiment, the preparation method of the hemolysin for blood analysis comprises the following steps:

[0035] Step S01, weighing 0.5 g of sodium octadecyl sulfate, 0.02 g of ethanol, 2 g of citric acid, 1 g of sodium chloride, 5.1 g of potassium chloride, and 0.1×10 sodium bicarbonate -3 g, standby;

[0036] Step S02, adding 0.5 g of sodium octadecyl sulfate, 2 g of citric acid, 1 g of sodium chloride, and 5.1 g of potassium chloride obtained in step S01 into 600 g of water for full dissolution to obtain an initial mixture;

[0037] Step S03, adding 0.02 g of ethanol to the primary mixed solution prepared in step S02, and then adding 390 g of water to make the volume to 1 liter, to prepare a premixed solution;

[0038] Step S04, adding sodium bicarbonate 0.1×10 -3 g to the premixed solution prepared in step S03, the pH value of the premixed solut...

Embodiment 2

[0044] A reagent for blood analysis comprising hemolysin and staining fluid for blood analysis.

[0045] In this embodiment, the hemolysin used for blood analysis per liter includes the following components: 0.8 g of sodium octadecyl sulfate, 0.04 g of ethanol, 2.5 g of citric acid, 3 g of sodium chloride, and 5.2 g of potassium chloride g. Sodium bicarbonate 0.3×10 -3 g.

[0046] In this embodiment, the preparation method of the hemolysin for blood analysis comprises the following steps:

[0047] Step S01, weighing 0.8 g of sodium octadecyl sulfate, 0.04 g of ethanol, 2.5 g of citric acid, 3 g of sodium chloride, 5.2 g of potassium chloride, and 0.3×10 sodium bicarbonate -3 g, standby;

[0048] Step S02, adding 0.8 g of sodium octadecyl sulfate, 2.5 g of citric acid, 3 g of sodium chloride and 5.2 g of potassium chloride obtained in step S01 into 600 g of water and fully dissolving to obtain an initial mixture ;

[0049] Step S03, adding 0.04 g of ethanol to the primary ...

Embodiment 3

[0056] A reagent for blood analysis comprising hemolysin and staining fluid for blood analysis.

[0057] In this embodiment, the hemolysin used for blood analysis per liter includes the following components: 1.0 g of sodium octadecyl sulfate, 0.06 g of ethanol, 2.8 g of citric acid, 7 g of sodium chloride, and 5.4 g of potassium chloride g. Sodium bicarbonate 0.6×10 -3 g.

[0058] In this embodiment, the preparation method of the hemolysin for blood analysis comprises the following steps:

[0059] Step S01, weighing 1.0 g of sodium octadecyl sulfate, 0.06 g of ethanol, 2.8 g of citric acid, 7 g of sodium chloride, 5.4 g of potassium chloride, and 0.6×10 sodium bicarbonate -3 g, standby;

[0060] Step S02, adding 1.0 g of sodium octadecyl sulfate, 2.8 g of citric acid, 7 g of sodium chloride and 5.4 g of potassium chloride obtained in step S01 into 600 g of water and fully dissolving them to obtain an initial mixture ;

[0061] Step S03, adding 0.06 g of ethanol to the pri...

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Abstract

The invention discloses hemolysin for blood analysis and a preparation method thereof and a reagent. The hemolysin comprises octadecyl sodium sulfate, ethanol, citric acid, sodium chloride, potassiumchloride and sodium bicarbonate. With the above technical scheme, the hemolysin has the advantages of simple preparation, high detection precision and wide application range.

Description

technical field [0001] The invention relates to the technical field of blood analysis, in particular to a hemolysin for blood analysis, a preparation method and a reagent thereof. Background technique [0002] Blood cell analysis is of great value for scientific research, disease prevention, diagnosis and treatment, from the initial study of cell shape, size, quantity to the most recent antigenic composition on the cell surface. Traditional cellular immunity and non-specific immune function detection techniques cannot perform multi-parameter and high-sensitivity analysis on the size, shape, plasma membrane, and internal structure of target cells at the single-cell level. Flow cytometry can quickly measure and sort , store, and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in liquid, and can sort out specified cell subpopulations according to the pre-selected parameter range, which is currently used in immunol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N15/14G01N33/50
CPCG01N1/30G01N15/1434G01N33/5094G01N2015/1006G01N2015/011G01N2015/012G01N2015/016
Inventor 李忠亮
Owner 四川恒健生物科技有限公司
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