Nicotine liquid culture medium, application and activation fermentation device

Abstract

The invention relates to a nicotine liquid culture medium, application and an activation fermentation device. A processing method of the nicotine liquid culture medium comprises the following steps: 1) pretreatment and impurity removal: standing nicotine liquid in a water tank, and removing impurities through natural sedimentation; 2) pH value adjustment: extracting supernatant, and adjusting thepH value to 6-7 with ammonia water; 3) sterilization: sterilizing at 121 DEG C for 15-30 minutes; and 4) nutrition regulation: regulating the contents of amino acids, proteins and sugars according tothe type of the strain to be cultured, and storing the culture medium under aseptic conditions for later use. The culture medium production method is scientific and reliable, the quality is controllable during the production process, the quality is stable and balanced, the processed culture medium is rich in nutrients and low in price, the culture medium is used for culturing strains, the targetsare high, strain propagation is fast, and infectious microbes are not prone to breeding. The nicotine liquid culture medium is rich in nutrients, the sugar content is up to 8%, the crude protein content is up to 12%, and the amino acids are balanced and comprehensive.

Description

technical field [0001] The invention belongs to the technical field of a nicotine liquid culture medium, its application and formula, its application method and the structure of an activation fermentation device. Background technique [0002] The currently used culture medium is glucose+peptone+vitamins+minerals (magnesium, potassium, zinc, etc.), which are costly and prone to the growth of miscellaneous bacteria such as molds, and it is difficult to achieve directional culture of bacteria. The unique nicotine in the nicotine liquid can inhibit the growth of mold. During the processing of the culture medium, due to too many factors in the environment, sterilization process, preparation process and human factors, the cost of the culture medium is high, it is easy to breed mold and other miscellaneous bacteria, it is difficult to achieve directional culture of bacteria, and the culture medium is easy to deteriorate, difficult to save. [0003] During the activation process o...

AI Technical Summary

Problems solved by technology

During the processing of the culture medium, due to too many factors in the environment, sterilization process, preparation process and human factors, the cost of the culture medium is high, it is easy to breed mold and other miscellaneous bacteria, it is difficult to achieve directional culture of bacteria, and the culture medium is easy to deteriorate, difficult to save

[0003] During the activation process of the existing strains, the strains need to be manually activated in batches, the operation is cumbersome, and the activation efficiency is low. Use, poor protection against activated bacteria

During the activation process, some fungi will produce gas. If it cannot be discharged in time, it will cause production safety accidents. After fermentation, the activated bacteria liquid needs to be transferred and inoculated into the raw materials to be fermented. In the existing method, manual spraying is required, which requires a large amount of work. The quantity is inaccurate, resulting in different fermentation effects of each batch of fertilizer

Images