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Pesticide for preventing and controlling cotton bollworms by nuclear polyhedrosis viruses as well as preparation method and application thereof

A technology of nuclear polyhedron and cotton bollworm, applied in the direction of botanical equipment and methods, applications, insecticides, etc., can solve the problems of no control, etc., and achieve the effect of reducing harm, killing insects quickly and effectively

Active Publication Date: 2020-10-02
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nuclear polyhedrosis virus is the earliest commercial virus biopesticide, and it is an important pathogenic microorganism of lepidopteran insects. The feces and dead insects of diseased insects can infect other larvae, and can also be passed on to offspring through eggs, but it is suitable for children under the third instar The young larvae have almost no control effect on the mature cotton bollworm above the 3rd instar, especially the 6th instar

Method used

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  • Pesticide for preventing and controlling cotton bollworms by nuclear polyhedrosis viruses as well as preparation method and application thereof
  • Pesticide for preventing and controlling cotton bollworms by nuclear polyhedrosis viruses as well as preparation method and application thereof
  • Pesticide for preventing and controlling cotton bollworms by nuclear polyhedrosis viruses as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A pesticide for controlling nuclear polyhedrosis virus against cotton bollworm, the active ingredients of which are nuclear polyhedrosis virus and dew grass extract containing ecdysterone. The two are mixed according to a certain ratio.

[0026] In order to identify the effects of different proportions and concentrations of nuclear polyhedrosis virus and dew grass extract on the 6th instar cotton bollworm, experiments were carried out according to Table 1 to determine the survival rate. Divided into 12 groups, 50 heads in each group. The results are shown in Table 1. Wherein the preferred embodiment provided by the present invention, that is, the experimental method of the pesticide with one of the concentrations is as follows, and the experimental methods of other groups are similar and can be deduced according to those skilled in the art.

[0027] Dissolve 21 grams of dew grass extract containing 30% ecdysone (purchased from Xi'an Ruiying Biotechnology Co., Ltd.) wi...

Embodiment 2

[0047] A preparation method of a pesticide for controlling cotton bollworm by nuclear polyhedrosis virus, specifically comprising the steps of:

[0048] (1) Dissolve the dew grass extract containing 1.6-2.0Kg ecdysterone with water, and mix well to obtain the dew grass extract solution;

[0049] (2), the dew grass extract solution and containing 2.8 × 10 12 PIB-3.5×10 12 Solutions of PIB nuclear polyhedrosis virus were mixed to obtain the pesticide.

[0050] Application of a nuclear polyhedrosis virus pesticide in biological control of cotton bollworm. The application method per mu is as follows: the prepared nuclear polyhedrosis virus pesticide will be diluted to contain 1.6-2.0Kg ecdysone and 2.8×10 12 PIB-3.5×10 12 PIB nuclear polyhedrosis virus pesticide preparation is 500-1000mL, diluted to a certain volume, such as 20-30Kg, mixed and sprayed.

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Abstract

The invention belongs to the technical field of biological prevention and control of cotton bollworms; by adding ecdysterone-containing cyanotis arachnoidea extract into cotton bollworm nuclear polyhedrosis viruses, the effect of preventing and controlling aged cotton bollworms can be greatly improved, and meanwhile, the effect of preventing and controlling low-age cotton bollworms is better thanthat of a traditional method. The effect of preventing and treating the aged cotton bollworms by the nuclear polyhedrosis viruses can be remarkably enhanced according to the dosage of 5 mg of the cyanotis arachnoidea extract (30% ecdysterone) and 2.0 * 10 < 6 > PIB nuclear polyhedrosis viruses of each 6-year cotton bollworm. According to the pesticide provided by the invention, the death rate of larvae of 3 years old or below reaches 100% within 48 hours; the method has the advantages of quick effect and high efficiency for preventing and controlling the mature cotton bollworms, reduces the egg laying amount, has a remarkable effect for preventing and controlling the outbreak of the cotton bollworms in the next year, and fills the blank of no effective killing of the cotton bollworms at present.

Description

technical field [0001] The invention belongs to the technical field of biological control of cotton bollworm, and in particular relates to a pesticide for controlling cotton bollworm by nuclear polyhedrosis virus, a preparation method and application thereof. Background technique [0002] Cotton bollworm is a serious omnivorous pest. The mature 5-6 instar larvae of cotton bollworm enter the gluttony stage, which is the most harmful to crops. Cotton bollworm is also the species that lays the most eggs among insects. A single female moth can lay more than 3,000 eggs at most. If the control is not in place, a single overwintering female pupa can reproduce tens of millions of larvae in the coming year on average, and if there are too many eggs, it will grow up. Therefore, a small number of mature cotton bollworms that slip through the net will have a devastating effect. But at present, both chemical pesticides and biological pesticides are less effective in controlling mature c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/60A01N45/00A01P7/04
CPCA01N45/00A01N63/60
Inventor 柴连琴荆玉谱张林杰刘鹏飞孟景会张海燕王喆
Owner HENAN UNIVERSITY
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